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CAPRIN1 Transcriptionally Activated PLPP4 to Inhibit DOX Sensitivity and Promote Breast Cancer Progression.

Xiaorong Yuan1, Xuejie Yang2

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Cell Biochemistry and Biophysics
|November 18, 2024
PubMed
Summary

This study reveals that CAPRIN1 activates PLPP4, hindering doxorubicin (DOX) sensitivity and promoting breast cancer (BC) progression. Targeting PLPP4 offers a new strategy to improve DOX efficacy in BC patients.

Keywords:
Breast cancerCAPRIN1DOXPLPP4

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Phospholipid phosphatase 4 (PLPP4) is implicated in cancer cell dynamics.
  • The specific role of PLPP4 in breast cancer (BC) progression and doxorubicin (DOX) sensitivity is not well understood.

Purpose of the Study:

  • To investigate the role of PLPP4 in breast cancer progression.
  • To determine the effect of PLPP4 on doxorubicin (DOX) sensitivity in breast cancer cells.
  • To elucidate the regulatory relationship between PLPP4 and CAPRIN1 in breast cancer.

Main Methods:

  • Quantitative real-time PCR and western blotting were used to analyze PLPP4 and CAPRIN1 expression.
  • Functional assays (colony formation, EdU, Transwell, flow cytometry) assessed cellular behaviors.
  • CCK-8, in vivo xenograft models, RNA immunoprecipitation, and dual-luciferase reporter assays investigated DOX sensitivity and PLPP4-CAPRIN1 interactions.

Main Results:

  • PLPP4 expression was upregulated in BC tissues and cells.
  • Downregulation of PLPP4 suppressed BC cell proliferation, migration, and invasion, while enhancing apoptosis and DOX sensitivity.
  • CAPRIN1 was identified as a transcription factor that upregulates PLPP4, negatively impacting DOX sensitivity and promoting BC progression.

Conclusions:

  • CAPRIN1 transcriptionally activates PLPP4, inhibiting DOX sensitivity and promoting breast cancer progression.
  • Targeting PLPP4 presents a potential therapeutic strategy to enhance doxorubicin efficacy in breast cancer patients.