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Types of Reversible Electrodes

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For electrode reversibility to be maintained, all the reactants and products involved in the half-reaction must be present at the electrode. There are several types of reversible electrodes (half-cells).In metal-metal-ion electrodes, a metal balances electrochemically with a solution of its own ions. Examples are Cu2+|Cu and Zn2+|Zn. Metals that react with the solvent, like group 1 and most group 2 metals, which react with water, and zinc, which reacts with aqueous acidic solutions, cannot be...

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Synthesis and Performance Characterizations of Transition Metal Single Atom Catalyst for Electrochemical CO2 Reduction

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Published on: April 10, 2018

NADH-dependent CO₂ reductase on graphite for capacitive electrocatalytic interfacing mediated by solid-binding

J Shanthi Sravan¹, Hyeryeong Lee¹, Yuna Bang²

¹School of Environment and Energy Engineering, Gwangju Institute of Science and Technology (GIST), 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea; Research Center for Innovative Energy and Carbon Optimized Synthesis for Chemicals (inn-ECOSysChem), Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea.

Bioresource Technology

|November 18, 2024

Summary

Researchers engineered CO2 reductase (CR) with a graphite-binding peptide to improve electrode attachment for efficient CO2 electrosynthesis. This bio-hybrid approach enhances electron transfer for sustainable formate production.

Keywords:

Candida methylica Direct electron transfer Enzyme-electrode Formate dehydrogenase Solid-binding peptide

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Area of Science:

Biocatalysis
Bioelectrochemistry
Enzyme Engineering

Background:

NAD+/NADH-dependent CO2 reductase (CR) is crucial for CO2 conversion.
Efficient enzyme-electrode interfacing is key for direct electron transfer in biocatalysis.
Modifying enzymes with specific peptides can enhance their binding to electrode surfaces.

Purpose of the Study:

To investigate the impact of site-specific peptide fusion on the electrode binding of CR.
To evaluate the potential of engineered CR for efficient CO2 electrosynthesis via direct electron transfer.
To establish enzyme-electrode interfacing for enhanced CO2-based redox catalysis.

Main Methods:

Site-specific insertion of a graphite-binding peptide (Gr) into CR at N, C, and NC termini.
Evaluation of graphite surface-binding activity and electrode topography interaction.
Structural, enzymatic, and electrochemical characterizations of native and modified CRs.
Assessment of active site binding availability and adsorption/desorption capabilities.

Main Results:

Peptide modification influenced CR's binding affinity and interaction with graphite electrodes.
Engineered CR variants demonstrated altered electron transfer capabilities.
Site-specific fusion impacted active site accessibility and catalytic performance for CO2 reduction.
Successful CO2 reduction to formate was achieved using the enzyme-electrode complex.

Conclusions:

Peptide-modified CR enhances enzyme-electrode complex formation for efficient CO2 electrosynthesis.
Site-specific fusion is a viable strategy to optimize enzyme-electrode interactions for bioelectronic applications.
This work paves the way for upscaling enzymatic CO2 reduction using bio-hybrid systems.