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Bundle structures inside the deep cervical lymphatic vessels of mice.

Joonyoung Shin¹, Sungchul Kim^2,3,4

¹Institute for Global Rare Disease Network, Professional Graduate School of Korean Medicine, Wonkwang University, Iksan, 54538, Republic of Korea.

Scientific Reports

|November 18, 2024

Summary

Primo vessels (PVs) within deep cervical lymphatic vessels (dcLVs) were identified with unique structures and nm-sized pores. These findings suggest potential applications for targeted drug delivery via the lymphatic system.

Keywords:

AFM Bundle structures Deep cervical lymphatic vessels Drug delivery Primo vessels SEM

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Area of Science:

Lymphatic System Research
Vascular Biology
Microscopy and Imaging

Background:

The lymphatic system is vital for immunity and waste removal.
Primo vessels (PVs) within lymphatic vessels are poorly understood, especially in deep cervical lymphatic vessels (dcLVs).
Understanding PVs in dcLVs is crucial, particularly their connection to meningeal lymphatics.

Purpose of the Study:

To identify and characterize primo vessels (PVs) within deep cervical lymphatic vessels (dcLVs).
To investigate the structural features of PVs in dcLVs using advanced microscopy and staining techniques.
To explore the potential of PVs for targeted drug delivery applications.

Main Methods:

Utilized Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM).
Employed Alcian Blue (AB) and Hematoxylin and Eosin (H&E) staining.
Examined PVs within the deep cervical lymphatic vessels (dcLVs) of mice.

Main Results:

Identified distinct bundle structures of primo vessels (PVs) within dcLVs, composed of 3-5 primo subvessels (sub-PVs).
Determined sub-PV diameters to be approximately 5.4 ± 2.4 μm.
Confirmed nm-sized pores on sub-PVs via AFM, with observed rod-shaped nuclei.

Conclusions:

Primo vessels (PVs) possess unique structural characteristics within deep cervical lymphatic vessels (dcLVs).
The presence of nm-sized pores suggests potential for nanoparticle absorption and targeted drug delivery.
Further research is needed to elucidate the functional roles and therapeutic applications of PVs in lymphatic regions.