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miPEP31 alleviates sepsis development by regulating Chi3l1-dependent macrophage polarization
Yu Zhou1, Yuan Yuan1, Xuanqi Yao1
1Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China.
Biology Direct
|November 19, 2024
Summary
MicroRNA-31 (miR-31) levels are elevated in sepsis patients, particularly non-survivors. Suppressing miR-31 protects against sepsis by promoting beneficial macrophage polarization and reducing inflammation, suggesting miPEP31 as a potential therapy.
Area of Science:
- Immunology
- Molecular Biology
- Sepsis Pathophysiology
Background:
- Sepsis involves organ dysfunction due to an imbalanced immune response to infection.
- MicroRNAs (miRNAs) regulate biological processes, but miR-31's role in sepsis is unclear.
- Understanding miR-31's function is crucial for developing sepsis treatments.
Purpose of the Study:
- To investigate the role of miR-31 in sepsis immunopathology.
- To assess miR-31 as a potential sepsis biomarker.
- To evaluate the therapeutic potential of targeting miR-31 in sepsis.
Main Methods:
- Measured hsa-miR-31-5p levels in sepsis patients and healthy controls.
- Utilized a caecal ligation and puncture (CLP) sepsis model in mice.
- Investigated miR-31's effect on macrophage polarization (via Chi3l1) and therapeutic potential of miPEP31.
Main Results:
- Elevated hsa-miR-31-5p in sepsis patients, higher in non-survivors.
- mmu-miR-31-5p inhibited M2 macrophage polarization by downregulating Chi3l1.
- miPEP31 treatment reduced mortality, organ injury, and inflammation in a murine sepsis model.
Conclusions:
- miR-31 suppression is protective in sepsis, enhancing host defense via Chi3l1 upregulation.
- miPEP31 demonstrates significant therapeutic potential for sepsis treatment.
- miR-31 emerges as a key regulator in sepsis and a target for novel therapies.
