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Updated: Jun 7, 2025

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Published on: June 24, 2021
APAtizer: a tool for alternative polyadenylation analysis of RNA-Seq data
Bruno Sousa1,2,3, Maria Bessa1,2, Filipa L de Mendonça1,2
1Gene Regulation Group, i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, 4200-135, Portugal.
Summary:
APAtizer is a tool designed to analyze alternative polyadenylation events on RNA-sequencing data. The tool handles different file formats, including BAM, htseq, and DaPars bedGraph files. It provides a user-friendly interface that allows users to generate informative visualizations, including Volcano plots, heatmaps, and gene lists. These outputs allow the user to retrieve useful biological insights such as the occurrence of polyadenylation events when comparing two biological conditions. In addition, it can perform differential gene expression, gene ontology analysis, visualization of Venn diagram intersections, and correlation analysis.
Availability And Implementation:
Source code and example case studies for APAtizer are available at https://github.com/GeneRegulationi3S/APAtizer/.

