Primaquine Analysis in Pharmaceutical Formulation Using Multiple and Short-End Injections by Capillary Zone...

Area of Science:

• Analytical Chemistry
• Separation Science
• Pharmaceutical Analysis

Background:

• Accurate quantification of primaquine (PQN) in pharmaceutical formulations is crucial for quality control.
• Existing analytical methods may require optimization for speed and efficiency.

Purpose of the Study:

• To develop and validate novel capillary electrophoresis methods for primaquine determination in tablets.
• To compare the performance of multiple-injection capillary zone electrophoresis (MI-CZE) and short-end injection CZE (SEI-CZE).

Main Methods:

• Development of background electrolyte (BGE) using effective mobility versus pH curves.
• Optimization of electrophoretic conditions using experimental designs and chemometric tools.
• Implementation of MI-CZE with a spacer electrolyte for enhanced separation and SEI-CZE for rapid analysis.

Main Results:

• MI-CZE achieved baseline resolution with sample injection six times in under 3 minutes.
• SEI-CZE provided a rapid migration time of 0.6 minutes for PQN.
• Both methods demonstrated satisfactory validation parameters for routine quality control.

Conclusions:

• Developed MI-CZE and SEI-CZE methods are suitable for the routine quality control analysis of primaquine in tablets.
• These methods offer efficient and accurate determination of primaquine.
• Chemometric tools proved valuable in optimizing electrophoretic conditions.