Development of a new genotype-phenotype linked antibody screening system
Takashi Watanabe1, Hikaru Hata2, Yoshiki Mochizuki1
1Laboratory for Integrative Genomics, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.
Elife
|November 19, 2024
Summary
We developed a rapid method for screening recombinant monoclonal antibodies using a novel dual-expression vector system. This technique enables efficient isolation of high-affinity antibodies for therapeutic and diagnostic applications within seven days.
Area of Science:
- Biotechnology
- Immunology
- Molecular Biology
Background:
- Recombinant antibodies are crucial for disease therapy and diagnosis.
- Current recombinant antibody screening methods are limited by delayed screening post-production and challenges in expressing paired heavy and light chains.
Purpose of the Study:
- To introduce a streamlined method for rapid screening of recombinant monoclonal antibodies.
- To overcome limitations of existing antibody screening techniques.
Main Methods:
- Development of a Golden Gate-based dual-expression vector for simultaneous heavy and light chain expression.
- In-vivo expression of membrane-bound antibodies.
- Rapid isolation of influenza cross-reactive antibodies from immunized mice.
Main Results:
- Successful demonstration of rapid isolation of high-affinity, influenza cross-reactive antibodies.
- Isolation achieved within 7 days, significantly faster than conventional methods.
- The system effectively addresses challenges in paired chain expression.
Conclusions:
- The Golden Gate-based dual-expression system offers a highly efficient method for recombinant antibody screening.
- This approach accelerates the discovery of therapeutic and diagnostic antibodies, particularly relevant for pandemic preparedness.
