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miRNA-199b-5p suppresses of oral squamous cell carcinoma by targeting apical-basolateral polarity via Scribble/Lgl

  • 0Department of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, Gangneung-si, Gangwon-do 25457, Republic of Korea.
Molecular Therapy. Nucleic Acids +

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November 19, 2024

|

November 19, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Scribble protein loss promotes oral cancer metastasis by disrupting cell polarity. Hypoxia and miR-199b-5p influence this process, suggesting Scribble

Area Of Science

  • Oncology
  • Cell Biology
  • Molecular Biology

Background

  • Scribble protein is crucial for epithelial cell polarity and acts as a tumor suppressor.
  • Oral squamous cell carcinoma (OSCC) metastasis is poorly understood, hindering effective treatment.
  • Investigating OSCC metastasis mechanisms is vital for improving patient prognosis.

Purpose Of The Study

  • To elucidate the role of Scribble in OSCC metastasis.
  • To identify key molecular players regulated by Scribble in OSCC.
  • To explore the influence of microenvironmental factors like hypoxia on Scribble function in OSCC.

Main Methods

  • Investigated Scribble's effect on apical-basolateral polarity in OSCC cells.
  • Analyzed the expression and localization of lethal giant larvae 1 and E-cadherin.
  • Examined the role of hsa-miR-199b-5p and hypoxia in Scribble regulation.

Main Results

  • Scribble knockdown disrupted OSCC cell polarity by downregulating lethal giant larvae 1 and E-cadherin.
  • Scribble localization was impaired under hypoxic conditions.
  • hsa-miR-199b-5p maintained Scribble localization in OSCC cells, even under hypoxia.

Conclusions

  • Scribble acts as a tumor suppressor in OSCC, regulating cell polarity and adhesion.
  • Scribble's function in OSCC metastasis is modulated by the hypoxia-induced microenvironment via miR-199b-5p.
  • Targeting Scribble or miR-199b-5p may offer therapeutic strategies for OSCC.

Keywords:

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