Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

81.1K
Overview
81.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing Gypsum Plaster with Encapsulated Fischer-Tropsch Paraffin Wax as a Phase-Change Additive for Broad-Range Thermal Energy Storage.

Polymers·2026
Same author

Architecture-Dependent Disintegration Temperature of Electrospun PNIPAM-Based Scaffolds.

ACS omega·2026
Same author

Phase Transitions of P(NIPAM-<i>g</i>-PLA) Copolymers in the Injectable Hydrogel Design.

The journal of physical chemistry. B·2026
Same author

Engineering of Extracellular Vesicles for Targeted Delivery of Prodigiosin.

Biotech (Basel (Switzerland))·2026
Same author

Thermodynamic Inhibition of Carbon Dioxide Hydrate with Magnesium Chloride and Methanol: Comparative Phase Equilibrium and PXRD Study.

International journal of molecular sciences·2026
Same author

Nanoarchitectonics in Materials Science, Second Edition.

Materials (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jun 7, 2025

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
10:21

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

10.0K

Atomic force microscopy for characterization of decellularized extracellular matrix (dECM) based materials.

Svetlana Batasheva1,2, Svetlana Kotova1, Anastasia Frolova1

  • 1Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation.

Science and Technology of Advanced Materials
|November 19, 2024
PubMed
Summary

Atomic force microscopy (AFM) is crucial for characterizing decellularized extracellular matrix (dECM) scaffolds. This technique evaluates mechanical properties and topography, essential for creating effective cell and tissue engineering materials.

Keywords:
Cell scaffoldacellular organs and tissuesbioartificialbioinksdecellularizationtissue engineering

More Related Videos

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels
06:12

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels

Published on: December 8, 2023

1.4K
Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
07:44

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Published on: March 3, 2023

1.3K

Related Experiment Videos

Last Updated: Jun 7, 2025

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
10:21

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

10.0K
Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels
06:12

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels

Published on: December 8, 2023

1.4K
Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
07:44

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Published on: March 3, 2023

1.3K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Nanotechnology

Background:

  • Cells interact with the extracellular matrix (ECM) for vital functions.
  • Replicating native ECM properties in artificial scaffolds is key for in vitro studies and tissue engineering.
  • Decellularized ECM (dECM) offers a biomimetic material for scaffold development.

Purpose of the Study:

  • To review the application of Atomic Force Microscopy (AFM) in characterizing decellularized ECM (dECM)-based materials.
  • To highlight the importance of evaluating scaffold stiffness for cell fate and biomaterial design.
  • To introduce flicker-noise spectroscopy (FNS) for quantifying dECM microstructure.

Main Methods:

  • Utilizing Atomic Force Microscopy (AFM) for simultaneous mechanical and topographical assessment of scaffolds.
  • Employing flicker-noise spectroscopy (FNS) for micro- and nanostructure quantification.
  • Reviewing existing literature on AFM applications in dECM material characterization.

Main Results:

  • AFM provides essential data on scaffold stiffness and topography, crucial for cell behavior.
  • AFM can assess the decellularization efficiency and ECM preservation.
  • FNS offers a method to quantify the micro- and nanostructure of dECM.

Conclusions:

  • AFM is an indispensable tool for characterizing dECM-based materials for tissue engineering.
  • Accurate characterization of dECM mechanical properties is vital for designing functional artificial cell environments.
  • AFM and FNS enable precise evaluation of dECM for biomimetic scaffold development.