Bumblebees under stress: Interacting effects of pesticides and heatwaves on colony development and longevity

Pollinator decline is linked to intensified agricultural practices, pathogens, climate change, and several other factors. We investigated the combined impact of heat and pesticide stress on food consumption, survival, and reproductive fitness of bumble bees. As climate change is expected to intensify heatwaves, we simulated a present-day and a future heatwave scenario (as expected in 50 years). In both scenarios, we exposed microcolonies to three widely used pesticides: azoxystrobin (fungicide), flupyradifurone, and sulfoxaflor (both insecticides)-mixed into pollen and nectar in field-realistic concentrations. We found that bees always consumed the least of sulfoxaflor-treated food, whereas consumption did not differ between other treatments or heatwave scenarios. Surprisingly, pesticide-stressed colonies performed slightly better in the future heatwave scenario in terms of reproductive fitness and survival. Sulfoxaflor consistently had the strongest negative effect, reducing survival rates, brood development, and food consumption, although effects were less severe in the future heatwave scenario.