Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

In-Plane Conductivity as a Descriptor of Apparent Durability of RuO<sub>2</sub> Anodes in PEM Water Electrolysis.

Nano letters·2026

Same author

Nano calligraphy via optical electro-aligning manipulation.

Microsystems & nanoengineering·2026

Same author

Surface Passivation and Aggregation-Enhanced Emission of Luminescent Copper Nanoparticles for Advanced Biochemical Sensors.

Analytical chemistry·2026

Same author

Light-Programmable Nanograspers for Rapid Nanoplastics Detection in Biological Fluids.

ACS nano·2026

Same author

Streamlined Digital Microfluidics-Mass Spectrometry Strategy for Extracellular Vesicle Enrichment and Lipid Profiling.

Analytical chemistry·2026

Same author

Metal-Organic Framework@Gold Nanoparticles Enables Point-of-Care Diagnostics To Have Comparable Analytical Performance to Chemiluminescent Immunoassays.

Analytical chemistry·2026

Same journal

Strategic Design and Engineering of CRISPR/Cas-Powered Sensing Platforms for Enhanced Nucleic Acid Detection.

ACS sensors·2026

Same journal

Broad-Temperature Polymerase in Nucleic Acid Amplification-Based Diagnostics: From Thermal Precision to Dynamic Conditions.

ACS sensors·2026

Same journal

Fluidic Lipid-Bilayer-Enhanced Iontronic Nanopore: Machine-Learning-Driven Ultrasensitive MicroRNA Detection in Cancer Diagnostics.

ACS sensors·2026

Same journal

Plant-Plant Communication for Systemic Acquired Resistance under Biotic Stress Spatiotemporally Tracked by an <i>In Situ</i> Surface-Enhanced Raman Spectroscopy Aerosol Spraying Analyzer.

ACS sensors·2026

Same journal

Modulating Electronic Structure via Bimetallic D<i>-</i>Band Engineering toward an Ultrasensitive Sensor Platform for Caffeic Acid in Food.

ACS sensors·2026

Same journal

Indiscriminate <i>T</i><i>rans</i>-Cleavage Activity of CRISPR/SuCas12a2 Enables Sensitive Detection of SARS-CoV-2.

ACS sensors·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 6, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Self-Interference Digital Optofluidic Genotyping for Integrated and Automated Label-Free Pathogen Detection.

Tianqi Zhou¹, Rongxin Fu^1,2,3,4, Jialu Hou^1,2

¹School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.

|November 19, 2024

Summary

This summary is machine-generated.

This study presents a novel optofluidic platform for rapid, automated pathogen detection using asymmetric direct solid-phase recombinase polymerase amplification (RPA). The system offers sensitive, specific, and label-free genotyping, advancing infectious disease diagnostics.

Keywords:

digital microfluidics microbial analysis optical biosensor optofluidic in vitro diagnosis recombinase polymerase amplification

More Related Videos

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Related Experiment Videos

Last Updated: May 6, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Area of Science:

Optofluidics
Molecular Diagnostics
Biotechnology

Background:

Pathogens cause millions of deaths annually, with traditional detection methods being slow and labor-intensive.
Current nucleic acid amplification techniques require expensive equipment and skilled personnel.
Existing isothermal methods like recombinase polymerase amplification (RPA) lack specificity and automation.

Purpose of the Study:

To develop a self-interferometric digital optofluidic platform for real-time, label-free, and automated pathogen genotyping.
To overcome the limitations of existing pathogen detection methods through integration of digital microfluidics and hyperspectral interferometry.

Main Methods:

Asymmetric direct solid-phase RPA integrated with digital microfluidics.
DNA monolayer detection using hyperspectral interferometry for label-free analysis.
Automated sample processing and real-time genotyping.

Main Results:

High sensitivity (10 CFU·mL^-1) and specificity (differentiating four Candida species).
Fully automated detection of Gram-negative bacteria within 50 minutes.
Simultaneous detection of four indices, demonstrating high throughput.

Conclusions:

The developed optofluidic platform enables rapid, specific, and sensitive pathogen detection without exogenous labeling.
This integrated approach represents a significant advancement in automated pathogen quantitation and diagnostics.
The platform offers a practical solution for timely pathogen detection and analysis in various environments.