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Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
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Stigmasterol and its esters encapsulated in liposomes: Characterization, stability, and derivative formation
Magdalena Rudzińska1, Katarzyna Cieślik-Boczula2, Anna Grygier1
1Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland.
Food Chemistry
|November 19, 2024
Summary
Liposomes encapsulating stigmasterol derivatives show varying stability under heat. Stigmasteryl oleate liposomes exhibited the best thermal stability, crucial for pharmaceutical applications.
Area of Science:
- Lipid-based drug delivery systems
- Phytosterols and their derivatives
- Thermal stability assessment
Background:
- Dipalmitoylphosphatidylcholine (DPPC) liposomes are widely used for drug delivery.
- Phytosterols like stigmasterol and its esters (myristate, oleate) are of interest for their therapeutic potential.
- Understanding the thermal stability of encapsulated compounds is vital for formulation development and storage.
Purpose of the Study:
- To prepare and characterize DPPC liposomes encapsulating free stigmasterol (ST), stigmasteryl myristate (ME), and stigmasteryl oleate (OE).
- To evaluate the thermal degradation and derivative formation of these encapsulated phytosterols at 60°C and 180°C.
- To assess the impact of phytosterol structure on liposome stability under thermal stress.
Main Methods:
- Liposome preparation using DPPC.
- Encapsulation of ST, ME, and OE within liposomes.
- Characterization via Transmission Electron Microscopy (TEM), FT-IR spectroscopy, zeta potential, and hydrodynamic diameter.
- Thermal stress testing at 60°C and 180°C.
- Quantification of phytosterol degradation and oxyphytosterol (SOP) formation.
Main Results:
- Successful preparation of DPPC liposomes with encapsulated phytosterols was confirmed.
- ST liposomes were smallest; ME and OE liposomes were similar in size.
- Degradation varied by structure: at 60°C, ST (11%) < OE (47%) < ME (58%); at 180°C, OE (51%) < ST (85%) < ME (90%).
- Significant formation of oxyphytosterols (SOP) occurred at 180°C, particularly in ST and ME liposomes.
Conclusions:
- Liposome encapsulation offers a method for delivering phytosterol derivatives.
- The structural differences in phytosterol esters significantly influence their thermal stability within liposomes.
- Stigmasteryl oleate demonstrates superior thermal stability compared to free stigmasterol and stigmasteryl myristate, making it a promising candidate for heat-sensitive applications.
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