Rat coagulation factor V purification and production of the monospecific antiserum

An original method was applied to purify rat factor V. The final preparation had a sp. act. of 45 U/ml for a 2500-fold purification with a yield of 43%. The final product is partially activated since it is 4.7-fold activable by RVV-VAE vs 6.3 in plasma. It can explain the presence of some of the four slightly stained additional bands found in SDS-electrophoresis. Finally, results of the purification suggest that rat factor V is a 338,000 single chain glycoprotein with a strong molecular asymmetry. A factor V deficient fraction was produced and used to adsorb an anti-factor V antiserum. This adsorbed antiserum was found monospecific against purified rat factor V.