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Optimizing conditions in online RPLC × SFC for the analysis of complex samples containing neutral compounds: Solving

Jason Devaux1, Mélanie Mignot2, Morgan Sarrut3

  • 1COBRA, Normandie Université, Université de Rouen, INSA de Rouen, CNRS, UMR 6014, IRCOF 1 rue Tesnière, Mont Saint Aignan 76821, France; Université de Lyon, Institut des Sciences Analytiques, UMR 5280 CNRS, 5 rue de la Doua, Villeurbanne 69100, France; International Joint Laboratory, iC2MC: Complex Matrices Molecular Characterization, TRTG, BP 27, Harfleur 76700, France.

Journal of Chromatography. A
|November 19, 2024
PubMed
Summary

Optimizing online reversed-phase liquid chromatography and supercritical fluid chromatography (RPLC × SFC) enhances complex sample analysis. Key parameters like mobile phase composition and injection volume were identified for improved peak shape and separation power.

Keywords:
Injection effectInjection solventMicroalgae bio-oilOnline RPLC × SFCValve configuration

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