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Gelatin-alginate hydrogel for near-field electrospinning assisted 3D and 4-axis bioprinting

  • 0Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea.
Carbohydrate Polymers +

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November 19, 2024

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November 19, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Researchers developed a novel gelatin-alginate hydrogel for advanced 3D bioprinting and near-field electrospinning. This cytocompatible material enables complex scaffold fabrication with enhanced shape fidelity and controlled properties for tissue engineering.

Area Of Science

  • Biomaterials Science
  • Tissue Engineering
  • 3D Bioprinting

Background

  • Developing advanced hydrogels is crucial for creating functional tissue scaffolds.
  • Existing hydrogels often lack the necessary mechanical properties or printability for complex 3D structures.
  • Gelatin and alginate are biocompatible polymers with potential for hydrogel formation.

Purpose Of The Study

  • To synthesize a novel gelatin-alginate hydrogel suitable for both near-field electrospinning and 3D bioprinting.
  • To investigate the hydrogel's printability, mechanical properties, and cytocompatibility.
  • To explore its potential for fabricating complex scaffolds for tissue regeneration.

Main Methods

  • Synthesis of a gelatin-alginate hydrogel using tannic acid as a crosslinker.
  • Characterization of rheological properties for controlled extrusion.
  • Utilizing near-field electrospinning-assisted 3D printing and four-axis printing techniques.
  • Secondary crosslinking with Ca2+ ions to enhance mechanical stability and degradation profile.

Main Results

  • The synthesized hydrogel demonstrated excellent shape fidelity and a self-standing height over 20 mm.
  • Successful multilayered and four-axis 3D printing of complex scaffolds was achieved.
  • Near-field electrospinning resulted in significant diameter reduction (up to 74%).
  • Secondary crosslinking improved mechanical properties, shape fidelity, and prolonged degradation up to 21 days with minimal tannic acid release.

Conclusions

  • The developed gelatin-alginate hydrogel is highly versatile for advanced fabrication techniques like near-field electrospinning and 3D bioprinting.
  • Its controllable mechanical properties, high cytocompatibility, and cell supportiveness make it promising for diverse applications.
  • Potential applications include complex tissue regeneration scaffolds, drug delivery systems, and flexible electronic devices.

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