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Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles
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Paclitaxel and myricetin encapsulated hemoglobin nanoparticles: characterization and application
Ishaleena Chowdhury1, Goutam Pramanik2, Aparna Dutta2
1Department of Environmental Sciences, Maulana Abul Kalam Azad University of Technology, Kolkata, India.
Journal of Biomolecular Structure & Dynamics
|November 20, 2024
Summary
Hemoglobin (Hb) nanoparticles effectively encapsulate hydrophobic drugs like paclitaxel (PTX) and mynoline (MYN) for cancer therapy. Optimized acidic conditions yield stable nanoparticles with enhanced drug delivery and apoptosis-inducing efficacy.
Area of Science:
- Nanotechnology in drug delivery
- Biomaterials for cancer therapy
- Protein-drug conjugation chemistry
Background:
- Hemoglobin (Hb) serves as a biocompatible carrier for hydrophobic drugs.
- Developing effective nanoparticle formulations is crucial for cancer treatment.
- Encapsulating hydrophobic drugs like paclitaxel (PTX) and mynoline (MYN) presents formulation challenges.
Purpose of the Study:
- To synthesize and characterize hemoglobin nanoparticles (Hb NPs) for encapsulating PTX and MYN.
- To optimize the acid denaturation method for Hb NP synthesis.
- To evaluate the efficacy of drug-loaded Hb NPs in cancer cells.
Main Methods:
- Synthesis of Hb NPs using an acid denaturation method.
- Characterization via spectroscopic (CD, FTIR) and electron microscopic (SEM) techniques.
- Particle size analysis using Dynamic Light Scattering (DLS) and binding affinity determination.
Main Results:
- Optimal Hb NP synthesis achieved at pH 5.0, indicated by maximum Hb denaturation.
- Spectroscopic and microscopic analyses confirmed successful drug encapsulation and distinct NP morphologies (tetrahedral for PTX-Hb, round for MYN-Hb).
- PTX-Hb NPs exhibited higher dose-dependent apoptosis-inducing efficacy in K562 cells compared to MYN-Hb NPs.
Conclusions:
- Hemoglobin nanoparticles are a viable platform for encapsulating hydrophobic anticancer drugs.
- The acid denaturation method provides an effective route for synthesizing drug-loaded Hb NPs.
- PTX-loaded Hb NPs show promising enhanced efficacy for cancer treatment applications.
