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Improvement of osteogenesis and antibacterial properties of a bioactive glass/gelatin-based scaffold using zoledronic acid and CM11 peptide

  • 0PhD Candidate, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Veterinary Research Forum : an International Quarterly Journal +

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November 20, 2024

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November 20, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

This study enhanced bioactive glass scaffolds with zoledronic acid (ZA) and CM11 peptide, improving both bone regeneration and antibacterial properties. The modified scaffolds effectively promoted osteogenic differentiation and combatted resistant bacteria.

Area Of Science

  • Biomaterials Science
  • Tissue Engineering
  • Nanotechnology

Background

  • Bioactive glass (BG) scaffolds are promising for bone regeneration but often lack sufficient antibacterial properties.
  • Enhancing BG scaffolds with osteoinductive and antimicrobial agents is crucial for effective bone defect treatment.

Purpose Of The Study

  • To investigate the combined effects of zoledronic acid (ZA) and CM11 peptide on the osteoinduction and antibacterial capabilities of bioactive glass/gelatin (BG/Gel) scaffolds.
  • To evaluate the potential of ZA and CM11 peptide-modified BG/Gel (PZ-BG/Gel) scaffolds for bone tissue engineering.

Main Methods

  • BG/Gel composite scaffolds were fabricated using the sol-gel method, incorporating specific concentrations of CM11 peptide and ZA.
  • Characterization involved Fourier transform infrared spectroscopy, scanning electron microscopy, disk diffusion tests, and ultra-violet spectroscopy for drug release.
  • Mesenchymal stem cell differentiation was assessed via MTT, calcium, alkaline phosphatase assays, and gene expression analysis (OCN, collagen I, BMP2, RUNX2).

Main Results

  • The PZ-BG/Gel scaffolds demonstrated significant antimicrobial activity against multi-drug-resistant *Pseudomonas aeruginosa* and *Staphylococcus aureus*.
  • Enhanced osteogenic differentiation of mesenchymal stem cells was observed on PZ-BG/Gel scaffolds compared to unmodified BG/Gel scaffolds.
  • Key osteoblast markers and related gene expressions were significantly upregulated in cells cultured on PZ-BG/Gel scaffolds.

Conclusions

  • The incorporation of zoledronic acid and CM11 peptide effectively enhances the osteoinductive and antibacterial properties of bioactive glass-based scaffolds.
  • PZ-BG/Gel composite scaffolds show great potential for applications in bone tissue engineering, offering dual functionality.
  • This approach provides a promising strategy for developing advanced biomaterials for treating bone defects and infections.

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