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Altered Expression of Circulating miR-223-3p in Patients with Idiopathic Recurrent Pregnancy Loss: A Case and Control
Parisa Ostad Hasanzadeh1, Saeid Ghorbian2, Jafar Mohseni3
1Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran.
Background:
Circulating microRNAs (miRNAs or miRs) are key regulators of a wide array of biological processes that aberrantly express under physiological as well as pathological conditions. This study aims to determine whether altered expression of circulating miR-223-3p in plasma is associated with an increased risk of idiopathic recurrent pregnancy loss (iRPL), and if it could be a novel non-invasive biomarker for diagnosis of iRPL.
Materials And Methods:
In this case and control study, Plasma samples were obtained from 60 women with a history of ≥ 2 consecutive miscarriages and 60 women with at least one full-term pregnancy and no history of miscarriage. The samples were used to assess the expression of circulating miR-223-3p by quantitative real-time polymerase chain reaction (qPCR) and determine the correlation between patient clinico-demographic parameters and miR-223-3p expression levels. We used receiver operating characteristic (ROC) curve analysis to evaluate the diagnostic accuracy of miR-223-3p for the diagnosis of iRPL.
Results:
There was significant up-regulation in miR-223-3p expression in patients with iRPL compared with healthy controls (P=0.002). No significant correlation was found between the expression level of miR-223-3p and the number of miscarriages in iRPL patients (P=0.344). ROC curve analysis revealed that the area under the curve (AUC) value for miR-223-3p was 0.658 [95% confidence interval (95% CI): 0.5590.757, P=0.002).
Conclusion:
These results suggest that a higher expression level of circulating miR-223-3p may be closely related with the increased risk of iRPL and possibly serve as a promising non-invasive diagnostic biomarker for iRPL.
Insights
Elevated circulating microRNA-223-3p (miR-223-3p) levels are linked to an increased risk of idiopathic recurrent pregnancy loss (iRPL). This finding suggests miR-223-3p may serve as a novel non-invasive biomarker for iRPL diagnosis.
Area of Science:
- Reproductive Biology
- Molecular Diagnostics
- Biomarker Discovery
Background:
- Circulating microRNAs (miRNAs) regulate biological processes and are implicated in various conditions.
- Aberrant miRNA expression is observed in physiological and pathological states.
- Idiopathic recurrent pregnancy loss (iRPL) is a condition with potential molecular underpinnings.
Purpose of the Study:
- To investigate the association between circulating miR-223-3p plasma levels and the risk of iRPL.
- To evaluate miR-223-3p as a potential non-invasive diagnostic biomarker for iRPL.
Main Methods:
- A case-control study involving 60 women with iRPL and 60 controls.
- Quantitative real-time polymerase chain reaction (qPCR) to measure plasma miR-223-3p expression.
- Receiver operating characteristic (ROC) curve analysis to assess diagnostic accuracy.
Main Results:
- Significantly higher miR-223-3p expression was observed in iRPL patients compared to controls (P=0.002).
- No significant correlation was found between miR-223-3p levels and the number of miscarriages.
- ROC analysis indicated an Area Under the Curve (AUC) of 0.658 for miR-223-3p in diagnosing iRPL (P=0.002).
Conclusions:
- Higher circulating miR-223-3p expression is associated with an increased risk of iRPL.
- Circulating miR-223-3p shows potential as a non-invasive diagnostic biomarker for iRPL.
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