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Published on: February 22, 2022
Cardiomyocyte-Specific FoxO1 Knockout Mice as Tools to Assess Cardiac Hypertrophy and Key Experimental Considerations
Kate L Weeks1,2,3, Julie R McMullen4,5,6,7,8
1Department of Anatomy and Physiology, The University of Melbourne, Parkville, VIC, Australia. Kate.weeks@unimelb.edu.au.
Abstract:
The generation of tissue-specific mouse models has provided a powerful strategy to understand the role of genes in specific tissues/cells of interest under control/basal conditions and in response to physiological and pathological stimuli. Here we describe the generation of cardiomyocyte-specific FoxO1 knockout mice using Cre-loxP technology to examine the role of FoxO1 for the induction of heart enlargement (cardiac hypertrophy) in settings of health and disease. We highlight breeding strategies for generating tissue-specific mouse models and key experimental considerations during characterization.
