Protocol for sorting cancer stem cells using a combination of anti-CD133 antibody and lectin cyanovirin-N
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.This study introduces a new method to isolate cancer stem cells (CSCs) by combining CD133 with specific glycan chains, improving CSC identification and sorting for cancer research.
Area Of Science
- Biochemistry
- Cancer Biology
- Glycobiology
Background
- CD133 is a common marker for cancer stem cells (CSCs).
- The N-glycan structure of CD133 presents ambiguity, limiting precise CSC isolation.
- Improved markers are needed for accurate CSC identification and therapeutic targeting.
Purpose Of The Study
- To develop a refined protocol for isolating cancer stem cells (CSCs).
- To enhance the specificity of CSC markers by combining CD133 with glycan chain information.
- To provide a method for more accurate sorting of CSCs from tumor samples.
Main Methods
- Purification and biotinylation of cyanovirin-N (CVN) proteins.
- Development of a protocol combining CD133 and α-1,2-high-mannose type glycan chains.
- Sorting of tumor cells expressing both CD133 and α-1,2-linked mannose (CD133+α-1,2-Man+).
Main Results
- A novel method for identifying and sorting CSCs was established.
- The combination of CD133 and α-1,2-high-mannose glycans provides a more specific CSC marker.
- The protocol enables precise isolation of CD133+α-1,2-Man+ cells.
Conclusions
- The developed protocol offers enhanced specificity for cancer stem cell isolation.
- Combining CD133 with specific glycan markers improves CSC identification accuracy.
- This method has potential applications in cancer research and therapy development.
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.