Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lipid Catabolism01:25

Lipid Catabolism

1
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
1
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

1
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
1
Fates of Pyruvate01:20

Fates of Pyruvate

8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K
Microbial Fermentation01:23

Microbial Fermentation

1
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1
  1. Home
  3. Research Domains
  5. Engineering
  7. Automotive Engineering
  9. Automotive Combustion And Fuel Engineering
  11. Long-chain Fatty Acids As Sole Carbon Source In Polyhydroxyalkanoates Production By Cupriavidus Necator H16.
  1. Home
  3. Research Domains
  5. Engineering
  7. Automotive Engineering
  9. Automotive Combustion And Fuel Engineering
  11. Long-chain Fatty Acids As Sole Carbon Source In Polyhydroxyalkanoates Production By Cupriavidus Necator H16.

Related Experiment Video

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids
06:45

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Published on: August 9, 2024

1.0K

Long-chain fatty acids as sole carbon source in polyhydroxyalkanoates production by Cupriavidus necator H16.

Florencia Ridella1, Ismael Marcet1, Manuel Rendueles1

  • 1University of Oviedo, Department of Chemical Engineering and Environmental Technology. Julián Clavería 8, Faculty of Chemistry, Oviedo, Spain.

Bioresource Technology
|November 20, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Oleic acid enhances polyhydroxyalkanoates (PHA) production from waste oils more effectively than other fatty acids. Tailoring fatty acid feedstock optimizes PHA yield and properties for sustainable bioplastics.

Keywords:
Bioprocess kineticsCarbon sourceMicrobial polymerVegetable oil

More Related Videos

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
20:28

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

14.1K
Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

20.7K

Related Experiment Videos

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids
06:45

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Published on: August 9, 2024

1.0K
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
20:28

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

14.1K
Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

20.7K

Area of Science:

  • Biotechnology
  • Polymer Science
  • Microbiology

Background:

  • Polyhydroxyalkanoates (PHA) are biodegradable polymers offering sustainable alternatives to conventional plastics.
  • Utilizing waste oils as a feedstock for PHA production is crucial for economic viability and environmental benefit.

Purpose of the Study:

  • To evaluate the impact of key fatty acids (palmitic, stearic, oleic, linoleic) from waste and fresh oils on PHA production by Cupriavidus necator H16.
  • To determine how different fatty acids and concentrations influence PHA yield, composition, thermal properties, and microbial growth.

Main Methods:

  • Cultivation of Cupriavidus necator H16 with varying concentrations (5 g/L and 15 g/L) of specific fatty acids for 168 hours.
  • Analysis of PHA production yield, monomer composition, and thermal characteristics.
  • Assessment of microbial viability under different fatty acid feeding conditions.

    • Main Results:

    • Oleic acid demonstrated the highest efficacy as a carbon source for PHA production, particularly at higher concentrations.
    • The type and concentration of fatty acids significantly altered PHA monomer composition and thermal properties.
    • Stearic acid incorporation led to increased 3-hydroxyvalerate and medium-chain length monomers.
    • Linoleic acid exhibited a detrimental effect on microbial viability, unlike other tested fatty acids.

    Conclusions:

    • Fatty acid selection is a critical factor for optimizing PHA production efficiency and tailoring polymer characteristics.
    • Findings support the strategic use of specific fatty acids from waste oils to enhance PHA yield and properties for diverse industrial applications.
    • Cupriavidus necator H16 shows potential for PHA synthesis using various fatty acids, with oleic acid being a preferred substrate.