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A protein structure-dependent fluorescent probe for hemoglobin monitoring and controllable imaging in living cells
Xian-Ting Yan1, Kai-Li Chang1, Zi-Bei Huang2
1School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
Abstract:
A novel protein structure-dependent non-covalent fluorescent probe, DDBM, was developed. It exhibited selective fluorescence "turn-off" responsiveness to bovine hemoglobin (BHb). This responsiveness depended on the interaction between the probe and BHb, with the methoxynaphthalene group significantly contributing to the sensitivity. Non-covalent interactions played a crucial role in stabilizing the binding of DDBM with BHb. DDBM demonstrated a robust anti-interference capability in its BHb responsiveness. Interestingly, the BHb responsiveness of DDBM could be modulated by ibuprofen. Additionally, DDBM exhibited favorable fluorescence enhancement sensitivity to bovine serum albumin (BSA), coupled with a robust anti-interference capability. These distinctive properties of DDBM enabled it to dynamically trace the metabolism of hemoglobin (Hb) and further achieve Hb-mediated precise controllable live cell imaging.
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