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Related Experiment Video

Updated: May 1, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
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Profiling CARD14 gene expression in Indian Psoriasis patients.

S Singh1, D Pradhan2,2, P Puri3,4

  • 1ICMR-National Institute of Pathology, New Delhi, 110029, India.

Scientific Reports
|November 20, 2024
PubMed
Summary

Caspase Recruitment Domain-containing family 14 (CARD14) gene expression is significantly up-regulated in Indian psoriasis patients. This finding offers new insights into the genetic factors contributing to psoriasis pathogenesis.

Keywords:
CARD14Genetic triggersKeratinocytesNF-kB signaling pathwayPSORS2

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Area of Science:

  • Genetics
  • Dermatology
  • Molecular Biology

Background:

  • Psoriasis is a chronic inflammatory skin condition with a complex genetic basis.
  • Genome-Wide Linkage Studies have identified several susceptibility loci for psoriasis.
  • Caspase Recruitment Domain-containing family 14 (CARD14) is a key regulator of the NF-kB signaling pathway, implicated in skin inflammation.

Purpose of the Study:

  • To investigate the role of CARD14 gene expression in Indian patients with plaque psoriasis.
  • To explore the potential functional consequences of CARD14 dysregulation in psoriasis pathogenesis.
  • To correlate CARD14 expression levels with disease severity in an Indian cohort.

Main Methods:

  • Quantitative Polymerase Chain Reaction (qPCR) was used to analyze CARD14 gene expression in lesional skin samples.
  • Immunohistochemistry (IHC) was employed for validation of CARD14 expression in different cell types.
  • Gene expression data from Western populations were used for comparative analysis.

Main Results:

  • A significant two-fold up-regulation of CARD14 expression was observed in lesional tissues of Indian psoriasis vulgaris patients compared to controls.
  • CARD14 expression was identified in dermal endothelial cells of psoriatic patients.
  • The findings were consistent with CARD14 expression patterns observed in Western populations.

Conclusions:

  • CARD14 up-regulation is significantly associated with psoriasis in the Indian population.
  • The study highlights CARD14's role in the pathogenesis of psoriasis, particularly its expression in dermal endothelial cells.
  • These findings contribute to understanding the genetic architecture and molecular mechanisms underlying psoriasis.