Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multi-organ spatial metabolomic atlas of exercising mice reveals neuronal Complex I as a convergent and sufficient axis for tau pathology reduction in PS19.

bioRxiv : the preprint server for biology·2026
Same author

Regulatory logic underlying neural crest contributions to the head versus the heart.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Hyperglycosylation is a metabolic driver of Alzheimer's disease.

Nature metabolism·2026
Same author

Pediatric epilepsy surgery: Global survey of referral and presurgical evaluation practices.

Epilepsia·2026
Same author

Maternal adiposity and inflammatory immune trajectories during pregnancy in women with HIV.

medRxiv : the preprint server for health sciences·2026
Same author

Metabolic Coherence of the Mouse Brain.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 23, 2026

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development
13:47

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Published on: April 3, 2013

12.9K

A temporal cortex cell atlas highlights gene expression dynamics during human brain maturation.

Christina Steyn1,2, Ruvimbo Mishi1,2, Stephanie Fillmore1,2

  • 1Division of Cell Biology, Department of Human Biology, University of Cape Town, Cape Town, South Africa.

Nature Genetics
|November 20, 2024
PubMed
Summary

This study maps 75 human brain cell subtypes in pediatric and adult temporal lobes using single-nucleus RNA sequencing. It reveals key gene expression changes during brain maturation, particularly in excitatory neurons linked to cognition.

More Related Videos

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
10:12

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Published on: August 9, 2017

8.1K
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
08:30

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

Published on: March 4, 2020

8.8K

Related Experiment Videos

Last Updated: Jun 23, 2026

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development
13:47

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Published on: April 3, 2013

12.9K
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
10:12

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Published on: August 9, 2017

8.1K
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
08:30

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

Published on: March 4, 2020

8.8K

Area of Science:

  • Neuroscience
  • Genomics
  • Developmental Biology

Background:

  • The human brain's postnatal development involves complex gene expression changes.
  • Bulk tissue analyses obscure cell-type-specific maturational dynamics.
  • Existing brain atlases often lack diversity and detailed cell-type resolution.

Purpose of the Study:

  • To create a comprehensive atlas of pediatric and adult human brain cell subtypes.
  • To identify genes and pathways that change during brain maturation.
  • To investigate cell-type-specific differences between pediatric and adult brains, including diverse ancestries.

Main Methods:

  • Single-nucleus RNA sequencing (snRNA-seq) on temporal lobe tissue.
  • Integration of pediatric and adult samples, including African ancestry.
  • Spatial transcriptomics for atlas validation.
  • Comparative analysis of gene expression in distinct cell subtypes.

Main Results:

  • A joint atlas of 75 distinct cell subtypes was constructed.
  • Significant differences in gene expression were identified between pediatric and adult cell subtypes.
  • Specific excitatory neuron subtypes (LTK, FREM) showed higher expression of cognition- and plasticity-related genes in pediatric samples.

Conclusions:

  • This study provides a high-resolution map of human brain cell subtypes during maturation.
  • It reveals critical molecular changes underlying brain development.
  • The findings contribute to a more inclusive understanding of brain cell diversity and function.