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Correlation between PDGF-BB and M1-type macrophage in inflammatory bowel disease: a case-control study
Zhiyun Fang1, Siwen Qu1, Xia Ji1
1The Second Hospital of Jiaxing, Jiaxing, 314000, China.
Background:
Inflammatory bowel disease (IBD) is a chronic disease in which macrophages play an important role in its pathogenesis. Platelet-derived growth factor-BB (PDGF-BB) secreted by macrophages is involved in the repair of vascular endothelial injury during inflammatory reactions.
Methods:
The expression levels of M1 macrophages and PDGF-BB in serum and colonic mucosa of 30 patients with Crohn's disease (CD) and 30 patients with ulcerative colitis (UC) were measured using enzyme-linked immunosorbent assays and immunohistochemistry. Logistic regression was used for univariate and multivariate analyses, and receiver operating characteristic curves were used to evaluate diagnostic value. Associations were evaluated using Spearman correlation analysis.
Results:
The expression of serum PDGF-BB and M1 macrophages with positive CXCL9 expression in patients with active-stage IBD [206.55(160.41,262.90)and 337.30(217.73,472.28) pg/ml] was higher than that in patients with remission stage [153.42(107.02,219.68)and 218.37(144.49,347.33)pg/ml] and controls [156.19(91.16,216.08)and 191.20(121.42,311.76)pg/ml](P < 0.05). The expression of PDGF-BB, CD86, and CXCL9 in the colon of patients with active-stage IBD [0.380(0.266,0.542) 0.663(0.480,0.591) and 0.564(0.378,0.765) /µm2] was higher than that in the remission stage [0.308(0.214,0.420), 0.376(0.206,0.591) and 0.413(0.275,0.570) /µm2] and controls [0.265(0.185,0.384), 0.416(0.269,0.534) and 0.497(0.415,0.642) /µm2] (P < 0.05). A positive correlation was observed between CD86 and PDGF-BB, and CXCL9 and PDGF-BB levels in patients with IBD (P < 0.05). CD86 and PDGF-BB in the colonic mucosa were independent risk factors for active IBD, and the area under the curve for their combined diagnosis was 0.754 (95%CI: 0.654-0.852, P < 0.05).
Conclusions:
PDGF-BB was associated with M1 macrophages and has a potential diagnostic value for active IBD.
Trial Registration:
Not applicable.
Insights
Platelet-derived growth factor-BB (PDGF-BB) and M1 macrophages are elevated in active inflammatory bowel disease (IBD). These markers show diagnostic potential for active IBD, aiding in disease management.
Area of Science:
- Gastroenterology
- Immunology
- Biomarker Discovery
Background:
- Inflammatory bowel disease (IBD) involves macrophages in pathogenesis.
- Platelet-derived growth factor-BB (PDGF-BB) aids vascular repair during inflammation.
Purpose of the Study:
- To investigate the role of PDGF-BB and M1 macrophages in IBD.
- To evaluate their diagnostic value for active IBD.
Main Methods:
- Enzyme-linked immunosorbent assays and immunohistochemistry used for 30 CD and 30 UC patients.
- Logistic regression and ROC curve analysis for diagnostic value assessment.
- Spearman correlation analysis for evaluating associations.
Main Results:
- Elevated serum and colonic PDGF-BB and M1 macrophage markers (CD86, CXCL9) in active IBD patients compared to remission and controls.
- Positive correlation between CD86, CXCL9, and PDGF-BB levels.
- CD86 and PDGF-BB in colonic mucosa are independent risk factors for active IBD.
Conclusions:
- PDGF-BB is associated with M1 macrophages in IBD.
- PDGF-BB and M1 macrophage markers show potential for diagnosing active IBD.
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