Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

2
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
2

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring bacteria-surface interactions with a fluorescent membrane tension probe.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Correlations of surface tension for mixtures of <i>n</i>-alkanes as a function of the composition: applicability and performance analysis of existing models.

Physical chemistry chemical physics : PCCP·2025
Same author

Real-Time Imaging of the Mechanobactericidal Action of Colloidal Nanomaterials and Nanostructured Topographies.

Small science·2025
Same author

Anti-Biofilm Perspectives of Propolis against <i>Staphylococcus epidermidis</i> Infections.

Biomolecules·2024
Same author

<i>In Situ</i> Single-Cell Bacterial Imaging Provides Mechanistic Insight into the Photodynamic Action of Photosensitizer-Loaded Hydrogels.

ACS applied materials & interfaces·2024
Same author

Min oscillations in bacteria as real-time reporter of environmental challenges at the single-cell level.

Open biology·2023

Related Experiment Video

Updated: Jun 7, 2025

Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging
07:34

Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging

Published on: September 2, 2021

3.2K

How Much Force is Needed to Kill a Single Bacterium?

Virginia Vadillo-Rodríguez1, Patricia Pedraz2, Cristina Flors2,3

  • 1Department of Applied Physics, University of Extremadura, Avda de Elvas s/n, Badajoz, 06006, Spain.

Small (Weinheim an Der Bergstrasse, Germany)
|November 21, 2024
PubMed
Summary
This summary is machine-generated.

Mechanobactericidal nanomaterials kill bacteria physically. Atomic force microscopy (AFM) nanoindentation quantifies forces needed to rupture bacterial cell walls, aiding understanding of these novel antimicrobial strategies.

Keywords:
atomic force microscopybacterial cell wallmechano‐bactericidalnanoindentationrupture force

More Related Videos

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
12:03

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Published on: April 18, 2019

26.1K
Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B
06:36

Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B

Published on: January 2, 2014

19.9K

Related Experiment Videos

Last Updated: Jun 7, 2025

Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging
07:34

Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging

Published on: September 2, 2021

3.2K
Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
12:03

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Published on: April 18, 2019

26.1K
Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B
06:36

Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B

Published on: January 2, 2014

19.9K

Area of Science:

  • Nanomaterial science
  • Microbiology
  • Biophysics

Background:

  • The rise of antibiotic resistance necessitates novel antimicrobial strategies.
  • Mechanobactericidal nanomaterials offer a physical alternative to traditional antibiotics.
  • Understanding the physical mechanisms of bacterial cell death is crucial for developing effective nanomaterials.

Purpose of the Study:

  • To review the application of atomic force microscopy (AFM) nanoindentation in quantifying bacterial cell wall rupture forces.
  • To discuss factors influencing measured rupture forces and complexities in modeling bacterial cell death.
  • To highlight the importance of pressure standardization for comparing results across studies.

Main Methods:

  • Utilizing atomic force microscopy (AFM) for nanoindentation of bacterial cell walls.
  • Quantifying the forces (in the nN range) required to induce cell rupture.
  • Analyzing the influence of experimental parameters on rupture force measurements.

Main Results:

  • Bacterial cell wall rupture forces typically range from nanonewtons (nN) to tens of nN.
  • Experimental variables like AFM tip properties, loading speed, and immobilization strategies affect rupture values.
  • Standardization of pressure is identified as critical for consistent and comparable results.

Conclusions:

  • AFM nanoindentation provides quantitative insights into bacterial mechanical fragility.
  • A deeper understanding of mechanobactericidal mechanisms can be achieved through standardized force measurements.
  • This knowledge is vital for designing advanced nanomaterials to combat bacterial infections.