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Updated: Jun 7, 2025

A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins
Published on: January 7, 2019
Wiring proton gradients for energy conversion
Xinchen Dai1, Cesare Berton2, Dong Jun Kim1
1School of Chemistry, University of New South Wales NSW 2025 Sydney Australia dongjun.kim@unsw.edu.au.
Abstract:
Light-switchable buffer solutions based on merocyanine photoacids can be used as efficient photoenergy harvesting systems. Varying the solvation environment of merocyanine photoacids in water-methanol mixtures allows one to carefully tune their photoacidity, relaxation kinetics, and solubility, opening up the possibility to install persistent pH gradients of approximately 4 pH units under 500 nm light. When interfaced between two electrodes and exposed to asymmetric light irradiation, these solutions can be photoactivated precisely both in space and time, generating open circuit voltages as high as 240 mV that can last hours under steady-state irradiation - an outcome that is akin the peak performance of biological transmembrane proton pumps.
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