Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.0K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.0K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

191
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
191
Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

1.8K
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
1.8K
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

149
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
149
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

194
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
194
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

762
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
762

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Site-Specific Assignments of C-H and C-D Vibrations in Gaseous 1-Butanol by High-Resolution Cavity-Enhanced Raman Spectroscopy.

The journal of physical chemistry. A·2026
Same author

Microwave and terahertz frequencies of O2 determined with saturated absorption spectroscopy near 763 nm.

The Journal of chemical physics·2025
Same author

Unprecedented accuracy in molecular line-intensity ratios from frequency-based measurements.

Science advances·2025
Same author

Vibrational Analysis Based on Cavity-Enhanced Raman Spectroscopy: Cyclohexane.

The journal of physical chemistry. A·2025
Same author

Postselection shifts the transition frequency of helium in an atomic beam.

Science advances·2025
Same author

Midinfrared Cavity-Enhanced Two-Photon Absorption Spectroscopy for Selective Detection of Trace Gases.

Analytical chemistry·2025

Related Experiment Video

Updated: Jun 7, 2025

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
08:22

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

Published on: August 6, 2018

6.8K

Profiling a pulsed molecular beam with cavity-enhanced absorption spectroscopy.

Zhuang Liu1, Qian-Hao Liu1, Cun-Feng Cheng1

  • 1State Key Laboratory of Molecular Reaction Dynamics, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China.

The Review of Scientific Instruments
|November 21, 2024
PubMed
Summary
This summary is machine-generated.

We developed a laser-based method to measure molecular beam density, crucial for chemical dynamics. This technique non-destructively determines the number of molecules in specific quantum states for advanced studies.

More Related Videos

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation
09:53

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation

Published on: October 30, 2012

12.9K
Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

8.9K

Related Experiment Videos

Last Updated: Jun 7, 2025

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
08:22

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

Published on: August 6, 2018

6.8K
Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation
09:53

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet VUV Synchrotron Radiation

Published on: October 30, 2012

12.9K
Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

8.9K

Area of Science:

  • Chemical Physics
  • Spectroscopy
  • Molecular Dynamics

Background:

  • Molecular beams are vital for chemical dynamics experiments.
  • Beam density significantly impacts reaction rates.
  • Accurate density measurements are needed for precise experimental control.

Purpose of the Study:

  • To present a novel method for measuring molecular beam density.
  • To demonstrate state-resolved density determination.
  • To enable non-destructive analysis of molecular beams.

Main Methods:

  • Utilized laser-locked cavity-enhanced absorption spectroscopy.
  • Measured the P(1) transition in the second overtone band of carbon monoxide (CO).
  • Analyzed absorption spectra to determine molecular number density.

Main Results:

  • Successfully measured the density of CO molecules in a specific quantum state within the molecular beam.
  • Demonstrated the capability of the spectroscopic method for state-resolved measurements.
  • Validated the non-destructive nature of the technique.

Conclusions:

  • The developed spectroscopic method accurately measures molecular beam density.
  • This technique allows for state-resolved characterization of molecular beams.
  • The method has broad applicability in chemical dynamics and collision studies.