Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA aptamers that inhibit binding to human interleukin-17A and interleukin-20.

RSC advances·2026
Same author

An RNA aptamer that shifts the reduction potential of metabolic cofactors.

Nature chemical biology·2022
Same author

Synthesis, characterization, crystal structure determination and biological screening of novel N-1 and C5 alkyl substituted scaffolds of pyrimidine.

European journal of medicinal chemistry·2015
Same author

Synthesis, biopharmaceutical characterization, antimicrobial and antioxidant activities of 1-(4'-O-β-D-glucopyranosyloxy-2'-hydroxyphenyl)-3-aryl-propane-1,3-diones.

European journal of medicinal chemistry·2011
Same author

Benign methodology and improved synthesis of 5-(2-chloroquinolin-3-yl)-3-phenyl-4,5-dihydroisoxazoline using acetic acid aqueous solution under ultrasound irradiation.

Ultrasonics sonochemistry·2011
Same author

Pharmacophores modeling in terms of prediction of theoretical physico-chemical properties and verification by experimental correlations of novel coumarin derivatives produced via Betti's protocol.

European journal of medicinal chemistry·2010

Related Experiment Video

Updated: Jul 13, 2026

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

11.8K

DNA Aptamers That Bind to Alginate Hydrogels.

Ali Parvez1, Dana A Baum1

  • 1Department of Chemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, Missouri 63103, United States.

ACS Biomaterials Science & Engineering
|November 21, 2024
PubMed
Summary

Novel DNA aptamers were developed to bind alginate hydrogels, acting as tethers for therapeutics. This innovation extends drug delivery duration in wound healing applications, improving therapeutic efficacy.

Keywords:
SELEXalginateaptamershydrogelsin vitro selection

More Related Videos

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

10.6K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

9.8K

Related Experiment Videos

Last Updated: Jul 13, 2026

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

11.8K
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

10.6K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

9.8K

Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Molecular Biology

Background:

  • Hydrogels are widely used in wound treatment due to their biocompatibility and healing promotion.
  • The porous nature of hydrogels leads to rapid diffusion of encapsulated therapeutics, limiting their effectiveness.
  • Aptamers, specific DNA/RNA sequences, offer a potential solution for controlled therapeutic release.

Purpose of the Study:

  • To develop DNA aptamers capable of binding to alginate hydrogels.
  • To investigate the potential of these aptamers as tethers for therapeutics to control diffusion.
  • To evaluate the efficacy of aptamer-tethered therapeutics in extended drug delivery.

Main Methods:

  • An in vitro selection method was employed to identify DNA aptamers targeting alginate hydrogels.
  • The binding affinity and specificity of selected aptamers to alginate hydrogels were assessed.
  • Aptamers were conjugated to bovine serum albumin (BSA) to test their functionality as tethers in diffusion studies.

Main Results:

  • Two DNA aptamers were identified that bind to alginate hydrogels across a concentration range of 0.5-2%.
  • These aptamers demonstrated specificity for alginate over agarose.
  • Aptamer-conjugated BSA exhibited significantly longer retention within hydrogels over a one-week period, irrespective of encapsulation or post-gelation introduction.

Conclusions:

  • Developed DNA aptamers effectively bind alginate hydrogels, serving as versatile tethers for therapeutic molecules.
  • These aptamers enable sustained drug delivery by preventing premature diffusion from hydrogel matrices.
  • The findings offer a flexible strategy for enhancing therapeutic outcomes in hydrogel-based wound treatments.