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Updated: Jun 7, 2025
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Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
Published on: March 23, 2022
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Mouse Small Intestinal Organoid Cultures
Lei Chen1,2, Xiaoting Xu3
1School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing, China. leichen@seu.edu.cn.
Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2024
Summary
We developed a novel coculture system integrating intestinal organoids with mesenchymal cells. This advanced model enhances physiological relevance for studying gut homeostasis, stem cell biology, and disease mechanisms.
Area of Science:
- Gastroenterology
- Stem Cell Biology
- Tissue Engineering
Background:
- The intestinal epithelium is vital for gut homeostasis and can be modeled using 3D intestinal organoids.
- Mesenchymal cells influence epithelial behavior via paracrine signaling and structural support.
- Current models lack the in vivo microenvironment's cellular complexity.
Purpose of the Study:
- To develop a coculture system combining intestinal organoids and mesenchymal cells.
- To create a more physiologically relevant in vitro model of the intestinal microenvironment.
- To facilitate the study of epithelial-stromal interactions.
Main Methods:
- Isolation of mouse intestinal crypts.
- Culture of three-dimensional (3D) intestinal organoids.
- Coculture of organoids with mesenchymal cells (direct or indirect contact).
Main Results:
- The coculture system preserves organoid 3D architecture.
- It enhances the physiological relevance of organoids by incorporating cellular complexity.
- The system supports long-term maintenance and experimental adaptability.
Conclusions:
- This coculture model provides a powerful tool for investigating intestinal stem cell biology, tissue regeneration, and disease.
- It enables detailed study of interactions between the intestinal epithelium and its stromal microenvironment.
- The system offers new insights into gut homeostasis and disease mechanisms.
