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Unveiling the Potential Prebiotic Effects of Edible Mushroom Pleurotus djamor During In Vitro Colonic Fermentation

Giuliane Moura Andrade1, Evandro Leite de Souza2, Jhonatan Rafael Zárate-Salazar3

  • 1Postgraduate Program in Nutrition Sciences, Health Sciences Center, Federal University of Paraíba, João Pessoa 58051-900, Brazil.

Journal of Agricultural and Food Chemistry
|November 21, 2024
PubMed

Insights

Edible mushrooms like Pleurotus djamor powder (MUS) show prebiotic potential. MUS positively altered gut bacteria, increasing beneficial microbes and producing health-promoting compounds during in vitro fermentation.

Area of Science:

  • Microbiology
  • Nutrition Science
  • Food Science

Background:

  • Edible mushrooms are recognized for their potential health benefits.
  • Modulation of the gut microbiota is crucial for overall health.
  • Pleurotus djamor (oyster mushroom) is a widely consumed edible mushroom.

Purpose of the Study:

  • To investigate the prebiotic effects of digested Pleurotus djamor powder (MUS).
  • To analyze the impact of MUS on human intestinal microbiota composition and function in vitro.
  • To identify bioactive compounds in P. djamor responsible for its effects.

Main Methods:

  • In vitro colonic fermentation model using digested Pleurotus djamor powder (MUS).
  • 16S rRNA gene sequencing to assess changes in bacterial populations.
  • Analysis of short-chain fatty acids (SCFAs) and pH.
  • 1H NMR spectroscopy to identify phenolic compounds and other metabolites.

Main Results:

  • MUS significantly increased the abundance of beneficial bacteria, including Lactobacillus spp./Enterococcus spp. and Bifidobacterium spp.
  • MUS reduced the levels of potentially harmful bacteria like Clostridium histolyticum.
  • Enhanced production of lactic acid and SCFAs, along with a decrease in pH, was observed.
  • Phenolic compounds (epicatechin, gallic acid, quercetin 3-glucoside) and essential amino acids were identified in MUS.

Conclusions:

  • Digested Pleurotus djamor powder exhibits significant prebiotic effects.
  • MUS promotes a healthier gut microbiota profile by modulating bacterial populations and metabolic output.
  • P. djamor is a potential source of functional ingredients for improving gut health.
Keywords:
edible mushroomfunctional foodmicrobiotaphenolic compoundsshort-chain fatty acids

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