Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Acid/Base Strengths and Dissociation Constants03:02

Acid/Base Strengths and Dissociation Constants

The relative strength of an acid or base is the extent to which it ionizes when dissolved in water. If the ionization reaction is essentially complete, the acid or base is termed strong; if relatively little ionization occurs, the acid or base is weak. There are many more weak acids and bases than strong ones. The most common strong acids and bases are listed below:
Relative Strengths of Conjugate Acid-Base Pairs02:29

Relative Strengths of Conjugate Acid-Base Pairs

Brønsted-Lowry acid-base chemistry is the transfer of protons; thus, logic suggests a relation between the relative strengths of conjugate acid-base pairs. The strength of an acid or base is quantified in its ionization constant, Ka or Kb, which represents the extent of the acid or base ionization reaction. For the conjugate acid-base pair HA / A−, the ionization equilibrium equations and ionization constant expressions are
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
Surface Active Agents01:27

Surface Active Agents

Surfactants, named for their behavior at interfaces, positively adsorb at the interfaces of two phases, reducing interfacial tension. Their versatility as emulsifiers, detergents, and foaming agents stems from this ability. Surfactants, often termed amphiphiles, share the property of amphipathy, with molecules having both hydrophilic and hydrophobic portions. The hydrophilic part is called the head, and the hydrophobic part, including an elongated alkyl substituent, forms the tail.Surfactants...
Micelles01:30

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biotinylated Silatrane: Development Functional Organosilicon Biointerfaces for Molecular Detection.

ACS applied materials & interfaces·2026
Same author

Rationally Controlled Electropolymerization of Conjugated Polymers: Bridging Mechanistic Insight, In-Situ Probing, and Interface Design.

ACS central science·2026
Same author

Probing Biointerfaces with QCM‑D and Electrochemistry: Opportunities and Challenges toward Integrated EQCM‑D Biosensing.

ACS measurement science au·2026
Same author

Divergent effects of PLA2G7 on prostate cancer biochemical recurrence in European American and African American men.

Journal of the National Cancer Institute·2026
Same author

Runx2 mutation plays a key role in the development of scoliosis.

Innovation (Cambridge (Mass.))·2026
Same author

Real-Time <i>In Situ</i> Spectroscopic and Electrochemical Analysis of Ion-Water-Polymer Interactions at Functionalized PEDOT Interfaces.

Analytical chemistry·2025

Related Experiment Video

Updated: May 14, 2026

Concurrent Quantification of Cellular and Extracellular Components of Biofilms
10:18

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Published on: December 10, 2013

8.3K

Quantitatively Elucidating the Trade-Off between Zwitterionic Antifouling Surfaces and Bioconjugation Performance.

Pai-Jung Yang1, Yu-Ching Hsu2, Jie-Ren Li2

  • 1Department of Materials Science and Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.

Langmuir : the ACS Journal of Surfaces and Colloids
|November 21, 2024
PubMed
Summary

Zwitterionic materials like PEDOT-PC reduce protein fouling but also decrease peptide probe capture efficiency. This study quantifies this trade-off using antifouling surfaces for biosensing applications.

More Related Videos

A Method of Targeted Cell Isolation via Glass Surface Functionalization
10:40

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

9.4K
Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification
07:32

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

Published on: April 7, 2017

9.4K

Related Experiment Videos

Last Updated: May 14, 2026

Concurrent Quantification of Cellular and Extracellular Components of Biofilms
10:18

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Published on: December 10, 2013

8.3K
A Method of Targeted Cell Isolation via Glass Surface Functionalization
10:40

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

9.4K
Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification
07:32

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

Published on: April 7, 2017

9.4K

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Biosensor Technology

Background:

  • Zwitterionic materials offer excellent hydrophilicity, minimizing nonspecific biomolecule adsorption.
  • However, antifouling properties can impede specific target capture by immobilized probes.
  • Developing surfaces balancing antifouling and capture efficiency is crucial for biosensors.

Purpose of the Study:

  • To investigate the impact of zwitterionic (PEDOT-PC) content on antifouling properties and peptide-protein capture efficiency.
  • To model the relationship between antifouling characteristics and specific binding in a peptide-protein system.
  • To validate the utility of designed surfaces for biosensing.

Main Methods:

  • Fabrication of poly(3,4-ethylenedioxythiophene) (PEDOT) surfaces with varying phosphorylcholine (PC) and maleimide ratios.
  • Utilizing quartz crystal microbalance with dissipation (QCM-D) to monitor protein adsorption and binding kinetics.
  • Employing electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) for electrochemical characterization.

Main Results:

  • Increasing PEDOT-PC content enhanced antifouling properties, reducing nonspecific protein adsorption.
  • Higher PEDOT-PC concentrations significantly decreased the specific binding efficiency of calmodulin (CaM) to the peptide probe.
  • A quantitative equation was proposed to describe the observed binding behavior.
  • Electrochemical methods confirmed increased impedance with protein adsorption, validating surface utility.

Conclusions:

  • Zwitterionic PEDOT-PC surfaces effectively reduce protein fouling but compromise specific capture efficiency.
  • The study provides a quantitative understanding of the antifouling-capture efficiency trade-off.
  • The designed surfaces demonstrate practical utility for biosensing applications where fouling is a concern.