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OsAMT1.1 knockout-induced decrease in cadmium absorption and accumulation by rice related to cadmium

Guangxin Li¹, Yun Wang¹, Jingguang Chen²

¹College of Resources and Environmental, Henan Agricultural University, Zhengzhou 450046, PR China.

Ecotoxicology and Environmental Safety

|November 21, 2024

Summary

Nitrogen (N) application increases cadmium (Cd) accumulation in rice. Knocking out the ammonium transporter gene OsAMT1.1 reduces Cd uptake, offering strategies to lower heavy metal contamination in crops.

Keywords:

Cd absorption Cd accumulation Gene expression Nitrogen OsAMT1.1 Rice

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Area of Science:

Plant Biology
Environmental Science
Agricultural Science

Background:

Cadmium (Cd) is a toxic heavy metal accumulating in rice, a major food source, posing health risks.
Nitrogen (N) availability influences plant growth and heavy metal uptake, including Cd in crops.

Purpose of the Study:

To investigate the impact of nitrogen (N) application on cadmium (Cd) absorption and accumulation in rice grown in contaminated soil.
To elucidate the role of the ammonium transporter gene OsAMT1.1 in Cd uptake and translocation in rice.

Main Methods:

Potting experiments were conducted to assess the effects of varying N concentrations on rice growth and Cd accumulation.
Analysis of Cd content in different rice tissues was performed.
Gene expression analysis of Cd absorption-related genes (OsIRT1, OsNRAMP1, OsNRAMP5) and characterization of OsAMT1.1 knockout mutants were carried out.

Main Results:

Increased N concentrations significantly enhanced rice biomass and Cd accumulation in plant tissues.
OsAMT1.1 knockout mutants exhibited substantially reduced Cd absorption and accumulation compared to wild-type plants.
OsAMT1.1 knockout specifically decreased Cd influx in roots under ammonium-N conditions and altered Cd distribution, with increased culm content but reduced brown rice content.

Conclusions:

Nitrogen supply plays a critical role in regulating Cd content in rice.
The ammonium transporter OsAMT1.1 is a key factor in controlling Cd absorption and accumulation in rice.
Understanding the molecular mechanisms involving N and OsAMT1.1 provides insights for mitigating Cd contamination in rice.