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Updated: Jun 6, 2025

In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices

In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices

Published on: September 20, 2019

A consensus definition for deep layer 6 excitatory neurons in mouse neocortex.

Su-Jeong Kim¹, Travis A Babola¹, Kihwan Lee¹

¹Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205, USA.

Biorxiv : the Preprint Server for Biology

|November 22, 2024

Summary

This summary is machine-generated.

Defining deep layer 6b (L6b) neurons is crucial for understanding neocortical function. This study establishes a consensus definition for L6b neurons, differentiating them from other layer 6 neurons and clarifying their unique connectivity and stable electrophysiological properties.

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Last Updated: Jun 6, 2025

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Area of Science:

Neuroscience
Cell Biology

Background:

Neocortical function relies on precise cell-type definitions.
Deep layer 6 (L6b) neurons are implicated in thalamocortical interactions, brain state modulation, and neuropsychiatric diseases.
Previous definitions of L6b neurons lacked consensus, hindering research.

Purpose of the Study:

To establish a consensus definition for deep layer 6b (L6b) neurons.
To differentiate L6b neurons from other layer 6 neurons, such as layer 6 corticothalamic neurons (L6CThNs).
To characterize the developmental stability of L6b neuron electrophysiological properties.

Main Methods:

Comparative analysis of molecular, transcriptional, and morphological methods for L6b neuron identification.
Utilized transgenic mouse lines for cell-type specific labeling and analysis.
Electrophysiological recordings to assess intrinsic properties and developmental stability.

Main Results:

Identified a core population of L6b neurons based on integrated definition approaches.
Demonstrated that L6b neurons project ipsilaterally between cortical areas, distinct from L6CThNs that innervate the sensory thalamus.
Found that intrinsic electrophysiological properties of L6b neurons stabilize after the first postnatal week despite early developmental changes.

Conclusions:

Provides a consensus definition for L6b neurons, facilitating cross-study comparisons.
Clarifies the distinct connectivity of L6b neurons within the neocortex.
Establishes the developmental stability of L6b neuron electrophysiology, aiding future functional studies.