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Related Experiment Video

Updated: Jun 25, 2026

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae

Published on: February 6, 2018

Embryo movement is required for limb tendon maturation.

Rebecca A Rolfe¹, Ebru Talak Bastürkmen¹, Lauren Sliney¹

¹Zoology, School of Natural Sciences, Trinity College Dublin, University of Dublin, Dublin, Ireland.

Frontiers in Cell and Developmental Biology

|November 22, 2024

Summary

Tendon maturation requires dynamic muscle movement for proper collagen alignment and cell organization. Controlled mechanical stimulation is crucial for developing robust, load-bearing tendons.

Keywords:

cellular organization collagen fiber alignment embryonic movement muscle paralysis skeletal development tendon maturation yes-associated protein

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Published on: January 12, 2022

Area of Science:

Biomechanical Engineering
Developmental Biology
Tissue Engineering

Background:

Tendon maturation post-differentiation is poorly understood.
Embryonic tendon mechanical properties change significantly between E16-E18, influenced by embryo movement.

Purpose of the Study:

Profile late tendon development, focusing on collagen alignment, cell organization, and YAP pathway activity.
Investigate the impact of mechanical cues on tendon maturation by comparing static and no loading conditions.

Main Methods:

Analyzed collagen fiber alignment, cell organization, and YAP pathway activity during late chick embryonic development.
Utilized rigid (static loading) and flaccid (no loading) immobilization models to assess mechanical influences.

Main Results:

YAP signaling is active and responsive to movement in developing tendons.
Collagen fiber alignment increased with time and static loading.
Immobilization disrupted the organized, columnar cell structure and collagen deposition.

Conclusions:

Dynamic muscle-generated stimulation is essential for specific aspects of tendon maturation.
Understanding tendon development informs strategies for engineering improved load-bearing tissues.