Compartmentalised single-chain nanoparticles and their function

Single-chain nanoparticles (SCNPs) are generated by intramolecular collapse and crosslinking of single polymer chains, thus conceptually resembling the structures of folded proteins. Their chemical flexibility and ability to form compartmentalised nanostructures sized ∼1 nm make them perfect candidates for numerous applications, such as in catalysis and drug delivery. In this review we discuss principles for the design, synthesis and analysis of SCNPs, with a focus on their compartmentalised structures, highlighting our own previous work. As such compartments offer the potential to generate a specific nanoenvironment e.g. for the covalent and non-covalent encapsulation of catalysts or drugs, they represent a novel, exciting, and expanding research area. Starting from the architectural and chemical design of the starting copolymers by controlling their amphiphilic profile, the embedding of blocks-, or secondary-structure-mimetic arrangements, we discuss design principles to form internal compartments inside the SCNPs. While the generation of compartments inside SCNPs is straightforward, their analysis is still challenging and often demands special techniques. We finally discuss applications of SCNPs, also linked to the compartment formation, predicting a bright future for these special nanoobjects.