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Bacterial lipases and chronic blepharitis.

J M Dougherty, J P McCulley

    Investigative Ophthalmology & Visual Science
    |April 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Chronic blepharitis patients harbor more lipolytic coagulase-negative staphylococci (C-NS) strains, particularly those hydrolyzing cholesteryl oleate and behenyl oleate. These findings suggest a role for specific bacterial lipases in blepharitis pathogenesis.

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    Area of Science:

    • Ophthalmology
    • Microbiology
    • Dermatology

    Background:

    • Chronic blepharitis is an inflammatory condition affecting the eyelids.
    • Bacterial lipases may contribute to the pathogenesis of blepharitis by hydrolyzing lipids in meibomian secretions.

    Purpose of the Study:

    • To investigate the lipolytic activity of bacterial strains isolated from the eyelids and conjunctivae of patients with chronic blepharitis and normal individuals.
    • To determine if specific lipolytic bacteria are associated with different clinical forms of blepharitis.

    Main Methods:

    • Bacterial cultures were obtained from eyelids and conjunctivae of 36 normal individuals and 60 blepharitis patients.
    • Isolated strains, including coagulase-negative staphylococci (C-NS), Propionibacterium acnes, and Staphylococcus aureus, were tested for lipase activity against triglycerides, cholesterol esters, and fatty waxes using thin-layer chromatography.

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  • Lipase activity was quantified as the percentage of strains capable of hydrolyzing each substrate.

    • Main Results:

    • Staphylococcus aureus and Propionibacterium acnes showed consistent lipase production, with P. acnes unable to hydrolyze cholesteryl oleate.
    • Coagulase-negative staphylococci (C-NS) exhibited significant strain variability in lipase activity.
    • Patients with specific blepharitis types (mixed staphylococcal/seborrheic, meibomian seborrheic, secondary meibomitis, and meibomian keratoconjunctivitis) had a higher prevalence of C-NS strains capable of hydrolyzing cholesteryl oleate and behenyl oleate compared to normal individuals.

    Conclusions:

    • Certain blepharitis patient groups harbor a greater proportion of lipolytic C-NS strains.
    • The increased presence of C-NS strains with specific lipase activities (cholesteryl oleate and behenyl oleate hydrolysis) may play a role in the pathogenesis of distinct chronic blepharitis forms.
    • Further research is warranted to elucidate the precise mechanisms by which these lipolytic bacteria contribute to eyelid inflammation.