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Updated: Jun 6, 2025
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Study of the Gut Microbiome in Patients with Psoriatic Arthritis
Gustavo Moreira Amorim1,2,3, Gláucio Ricardo Werner Castro3,4, Sueli Carneiro5,6
1Division of Dermatology and Pathology, Federal University of Rio de Janeiro Faculty of Medicine, Clementino Fraga Filho University Hospital, Rio de Janeiro, Brazil.
Background:
Gut dysbiosis may play a role in immune-mediated diseases, such as psoriasis. There is a growing interest in understanding microbiome influence, with speculations around the importance of an altered gut microbiome linked to the progression to psoriatic arthritis in psoriasis. The objective of this study is to study the gut microbiome in patients with severe psoriatic disease with or without psoriatic arthritis.
Methods:
V3/V4 16S rRNA gene sequencing and bioinformatics analyses were performed with the total DNA extracted from the stool samples of 30 patients with psoriatic disease, 15 of whom had documented psoriatic arthritis.
Results:
We found differences in gut microbiome composition in psoriatic arthritis patients when looking for relative and especially differential abundances. Bacteroidaceae family (P = .02), Bacteroides genus (P=.02), and Bacteroides uniformis (P=.03) were more abundant in psoriatic arthritis patients on differential abundance, adjusted for each taxonomic level. However, the present study did not show significant differences in alpha or beta diversity.
Conclusion:
This study shows different patterns of gut microbiome composition in patients with psoriatic arthritis, with significant overexpression of the Bacteroides genus. This reinforces the microbiome as a field of interest in psoriasis. Nevertheless, it should be noted that some previously described findings related to lower diversity and different clustering between groups could not be demonstrated, probably due to the small number of patients. Additionally, it remains difficult to understand the magnitude of the gut microbiome influence. Is dysbiosis a cause or consequence of the disease? However, the microbiome deserves our attention, especially since it brings different opportunities for intervention through diet, prebiotics and probiotics, pretreatment analysis, prognosis, and even microbiome modulation and transplantation.
Insights
Psoriatic arthritis patients show altered gut microbiome composition, with increased Bacteroides genus. This highlights the gut microbiome
Area of Science:
- Microbiome research
- Immunology
- Dermatology
Background:
- Gut dysbiosis is implicated in immune-mediated diseases like psoriasis.
- Altered gut microbiome is suspected in the progression of psoriasis to psoriatic arthritis.
- Understanding the microbiome's influence on psoriatic disease is a growing area of interest.
Purpose of the Study:
- To investigate the gut microbiome composition in patients with severe psoriatic disease.
- To compare the gut microbiome between psoriatic disease patients with and without psoriatic arthritis.
Main Methods:
- Stool samples were collected from 30 patients with psoriatic disease.
- V3/V4 16S rRNA gene sequencing and bioinformatics analyses were performed.
- 15 patients had documented psoriatic arthritis.
Main Results:
- Significant differences in gut microbiome composition were observed in psoriatic arthritis patients.
- The Bacteroidaceae family, Bacteroides genus, and Bacteroides uniformis were more abundant in psoriatic arthritis patients.
- No significant differences in alpha or beta diversity were found between groups.
Conclusions:
- Psoriatic arthritis is associated with distinct gut microbiome patterns, notably an overexpression of the Bacteroides genus.
- The findings support the microbiome as a significant area of research in psoriasis.
- Further research is needed to clarify the causal role of dysbiosis and explore microbiome-targeted interventions.
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