Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

7.2K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.2K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

6.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.7K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

3.9K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Plantar pressure in athletes with chronic ankle instability during single-leg landings at different heights.

Frontiers in bioengineering and biotechnology·2026
Same author

Plantar pressure in athletes with chronic ankle instability during single-leg landings at different heights.

Frontiers in bioengineering and biotechnology·2026
Same author

Disruption of Ankle Control Strategies by Exercise-Induced Fatigue During Stair Descent in Athletes With Chronic Ankle Instability.

Archives of physical medicine and rehabilitation·2025
Same author

<i>TBRG4</i> as a prognostic biomarker and key regulator of cell cycle and EMT in lung cancer.

Biomolecules & biomedicine·2025
Same author

Contribution of fluorescence imaging to thoracoscopic anatomical segmentectomy: a multicenter propensity matching analysis.

Journal of thoracic disease·2024
Same author

Mn-Based Mullites for Environmental and Energy Applications.

Advanced materials (Deerfield Beach, Fla.)·2024

Related Experiment Video

Updated: Jun 6, 2025

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

7.5K

Unveiling the impact of C15orf48 on non-small cell lung cancer through NF-kappa B signaling.

Wei Wang1, Lei Zhang2, Ansheng Wang2

  • 1Department of Thoracic Surgery, Guangzhou Red Cross Hospital affiliated to Jinan University, Guangzhou, China; Department of Thoracic Surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu, China.

Biomolecules & Biomedicine
|November 22, 2024
PubMed
Summary
This summary is machine-generated.

The C15orf48 gene promotes non-small cell lung cancer (NSCLC) progression by increasing proliferation, invasion, and immune cell infiltration, potentially via the NF-κB pathway. Its upregulation correlates with poor prognosis in lung cancer patients.

More Related Videos

The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

111.4K
Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NF&#954;B Activity and Breast Cancer Stem Cells
13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

12.2K

Related Experiment Videos

Last Updated: Jun 6, 2025

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

7.5K
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

111.4K
Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NF&#954;B Activity and Breast Cancer Stem Cells
13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

12.2K

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The role of C15orf48 in non-small cell lung cancer (NSCLC) remains largely uncharacterized.
  • Understanding novel gene functions is crucial for developing targeted lung cancer therapies.

Purpose of the Study:

  • To elucidate the functional role of C15orf48 in NSCLC pathogenesis.
  • To investigate the molecular mechanisms underlying C15orf48's influence on NSCLC progression.

Main Methods:

  • Bioinformatics analyses (Oncomine, TCGA, PPI, GO, KEGG, GSEA) and immunohistochemistry.
  • In vitro cellular assays (viability, proliferation, invasion, apoptosis, adhesion) and in vivo xenograft models.
  • Western blotting and gene knockdown studies to assess protein expression and pathway involvement.

Main Results:

  • C15orf48 was significantly upregulated in NSCLC tissues, correlating with poor prognosis.
  • C15orf48 knockdown suppressed NSCLC cell proliferation, invasion, adhesion, and tumor growth, while enhancing apoptosis and immune cell infiltration.
  • GSEA identified the NF-κB signaling pathway as significantly affected by C15orf48 modulation.

Conclusions:

  • C15orf48 acts as an oncogene, promoting NSCLC progression.
  • C15orf48 may exert its effects through influencing immune cell infiltration and activating the NF-κB signaling pathway.
  • Targeting C15orf48 could represent a novel therapeutic strategy for NSCLC.