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Updated: Jun 6, 2025

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Detection of polyphenols in carob pods (Ceratonia siliqua) from Southern Italy by a LC-HRMS method
Calogero Alfano1, Elisa Maria Domenica Messina1, Gaetano Cammilleri1
1Food Department, Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy.
Abstract:
The presence of polyphenols was examined in carob pods (Ceratonia siliqua) from Southern Italy 90 days after harvest by the validation of a reliable LC-HRMS method. A greater abundance of Apigenin (51490.22 ± 34399.16 μg/Kg) and Myricetin (24897.92 ± 108332.05 μg/Kg) compared to previous research works conducted in Mediterranean countries. Significant differences in the polyphenol content between sampling areas (p < 0.05) were observed, particularly differences in hesperidin and myricetin. These differences confirmed the role of geochemical and climatic conditions in the variation of polyphenol content. This study is a first regarding the phenolic content of carob pods from Southern Italy, confirming the presence of these substances even long after harvest and that carob pods are valuable sources of phenolic substances that may be useful in the prevention of diseases related to oxidative stress.
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