Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

87.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.5K
Sanger Sequencing01:57

Sanger Sequencing

752.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
752.9K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.1K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artificial intelligence enables scale, consistency, and rigor in forensic identity inference.

Croatian medical journal·2026
Same author

Terminology, retrieval bias, and field definition in forensic genetic genealogy.

Forensic science international. Synergy·2026
Same author

Large-scale analysis of DNA quantification metrics and SNP sequencing performance in unidentified human remains.

Forensic science international. Genetics·2026
Same author

Othram maps: a graph-powered platform for pedigree visualization and forensic intelligence.

Bioinformatics (Oxford, England)·2026
Same author

On the uncertainty associated with using a signal detection theory model to analyze data from forensic black-box studies.

Forensic science international·2026
Same author

Animal Species and Identity Testing: Developments, Challenges, and Applications to Non-Human Forensics.

Genes·2025

Related Experiment Video

Updated: Jun 6, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

24.3K

A molecular framework for enhancing quality control and sample integrity in forensic genome sequencing.

Steven A Bates1, Bruce Budowle2, Lee Baker1

  • 1Othram Inc., The Woodlands, TX, USA.

Forensic Science International. Genetics
|November 23, 2024
PubMed
Summary

Molecular etches are new synthetic DNA tags that improve forensic analysis by encoding sample information for better tracking and quality control. These molecular etches enhance DNA typing accuracy in genomic sequencing for crime scene investigations.

Keywords:
Internal Molecular Information SystemMassively Parallel SequencingMolecular EtchesMolecular TagsValidation Studies

More Related Videos

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

12.0K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.8K

Related Experiment Videos

Last Updated: Jun 6, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

24.3K
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

12.0K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.8K

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genomic Sequencing

Background:

  • DNA typing is crucial for forensic identification of evidence and individuals.
  • Current molecular tags in DNA typing (e.g., barcodes) aid sample tracking but don't fully leverage sequence variation for quality control.
  • Existing methods lack comprehensive internal systems for managing sample information throughout the forensic workflow.

Purpose of the Study:

  • Introduce molecular etches as a novel internal molecular information management system for DNA typing.
  • Enhance quality control in forensic DNA analysis by leveraging sequence variation.
  • Improve sample tracking, contamination detection, and authenticity verification in forensic workflows.

Main Methods:

  • Development of synthetic oligonucleotides termed 'molecular etches'.
  • Encoding detailed sample information within the molecular etches.
  • Validation of molecular etches in genomic sequencing applications for forensic DNA analysis.

Main Results:

  • Molecular etches successfully encode comprehensive sample information.
  • Demonstrated robustness of molecular etches in genomic sequencing.
  • Validation studies confirm their utility as a quality control tool.

Conclusions:

  • Molecular etches represent a significant advancement in forensic DNA analysis quality control.
  • They provide an integrated system for managing sample history, tracking, and verifying authenticity.
  • This technology enhances the reliability and interpretability of DNA typing results in forensic investigations.