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Related Experiment Video

Updated: Jun 6, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy
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Accelerated protein retention expansion microscopy using microwave radiation.

Meghan R Bullard1, Juan Carlos Martinez-Cervantes1, Norisha B Quaicoe1

  • 1Department of Biology, Georgetown University, Washington, DC 20057, USA.

Cell Reports Methods
|November 23, 2024
PubMed
Summary

Microwave-assisted expansion microscopy (ExM) significantly reduces protocol time from days to hours for tissue imaging. This optimized method maintains high nanoscale resolution and improves expansion efficiency in various organisms.

Keywords:
CP: ImagingCP: NeuroscienceDrosophila melanogasterXenopus laevis tadpolesexpansion microscopyimmunohistochemistrymicrowaveneural tissue

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Neuroscience

Background:

  • Expansion microscopy (ExM) enables nanoscale imaging (<70 nm) on conventional microscopes by isotropically expanding fixed tissues.
  • Standard ExM protocols are lengthy, often taking several days to complete.
  • Efficient ExM is crucial for high-resolution biological research.

Purpose of the Study:

  • To adapt and optimize the ExM protocol for faster processing of biological samples.
  • To investigate the efficacy of microwave (M/W) assistance in accelerating the ExM workflow.
  • To demonstrate the applicability of the M/W-assisted ExM (M/W-ExM) protocol across different species and tissue types.

Main Methods:

  • Adapted ExM for vibratome-sectioned Xenopus laevis tadpole brains.
  • Implemented microwave (M/W) irradiation to create a rapid M/W-ExM protocol.
  • Applied the M/W-ExM protocol to whole-mount Drosophila melanogaster brains.

Main Results:

  • Reduced ExM workflow time from days to hours for Xenopus brain tissue.
  • Achieved superior nanoscale resolution comparable to standard ExM.
  • Observed increased expansion magnitude with M/W-ExM.
  • Successfully decreased processing time for Drosophila IHC-ExM from 6 to 2 days.

Conclusions:

  • Microwave-assisted ExM (M/W-ExM) significantly accelerates the ExM protocol while maintaining high resolution.
  • M/W radiation appears to enhance the tissue expansion process.
  • The M/W-ExM protocol is adaptable to different organisms and tissue types, increasing experimental efficiency.