Advanced design of target-driven self-powered sensor assisted by cascade catalytic strategy

Area of Science:

• Biomedical Engineering
• Biosensing Technology
• Nanotechnology

Background:

• MicroRNA-451 (miRNA-451) is a crucial disease biomarker.
• Existing detection methods often lack sensitivity, stability, or self-powering capabilities.
• Enzyme biofuel cells (EBFCs) offer a promising platform for self-powered biosensing.

Purpose of the Study:

• To develop a self-powered microsensor platform for sensitive and stable detection of miRNA-451.
• To integrate cascade catalysis and hybridization chain reaction (HCR) for signal amplification.
• To create a portable and intelligent biosensing system for disease monitoring.

Main Methods:

• Fabrication of a microsensor platform incorporating enzyme-like ZIF-8 nanocapsules with biological enzymes.
• Utilizing a cascade catalysis system to enhance electron transfer and sensor stability.
• Implementing a target-triggered hybridization chain reaction (HCR) for signal amplification.
• Employing enzyme biofuel cells (EBFCs) for self-powered operation.

Main Results:

• The microsensor platform demonstrated a wide linear range (0.5-1.0 fmol/L) and a low limit of detection (0.13 fmol/L).
• The system exhibited excellent selectivity, reproducibility, and stability under optimal conditions.
• The cascade catalysis and HCR strategies significantly improved detection sensitivity and performance.

Conclusions:

• The developed self-powered microsensor platform offers a sensitive, stable, and intelligent method for miRNA-451 detection.
• The integration of ZIF-8 nanocapsules, biological enzymes, and HCR provides a synergistic amplification effect.
• This platform holds potential for applications in basic biochemical research, clinical diagnostics, and environmental monitoring.