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A Quantitative Glycomics and Proteomics Combined Purification Strategy
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Recent Advances in Labeling-Based Quantitative Glycomics: From High-Throughput Quantification to Structural

Zicong Wang1, Jingwei Zhang2, Lingjun Li1,2,3,4

  • 1School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Proteomics
|November 24, 2024
PubMed
Summary

Recent labeling strategies enhance mass spectrometry (MS) analysis of glycans, improving quantification and structural elucidation for N-glycomics and O-glycomics. These advancements aid biomarker discovery and therapeutic applications.

Keywords:
N‐glycanO‐glycanderivatizationglycan structure elucidationsialylated glycans

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Area of Science:

  • Glycomics and Mass Spectrometry
  • Posttranslational Modifications (PTMs)

Background:

  • Glycosylation is a vital PTM involved in biological processes and disease.
  • Glycan structural complexity challenges MS-based quantitative analysis.

Purpose of the Study:

  • To review recent advancements in labeling strategies for N-glycomics and O-glycomics.
  • To focus on enhancing MS analysis sensitivity, specificity, and throughput.

Main Methods:

  • Categorization of labeling strategies into isotopic/isobaric labeling, structural elucidation methods, and ionization/detection enhancement techniques.
  • Review of advancements for MALDI-MS and CE-based glycan analysis.
  • Highlighting emerging trends in single-cell glycomics and bioinformatics tools.

Main Results:

  • Isotopic/isobaric labeling improves multiplexing capacity and glycan quantification throughput.
  • Novel methods aid structural elucidation of complex glycans, including sialylated and fucosylated types.
  • Labeling techniques enhance ionization efficiency, separation, and sensitivity for MS and CE analyses.

Conclusions:

  • Labeling strategies are critical for advancing glycan identification and quantification.
  • Developments expand understanding of glycan structures and functions.
  • These advancements offer new avenues for biomarker discovery and therapeutic applications.