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Electrohydrodynamic-Printed Dual-Triphase Microfibrous Scaffolds Reshaping the Lipidomic Profile for Enthesis Healing
Lang Bai1,2, Ayiguli Kasimu2, Shuai Wang1,3
1Department of Orthopaedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China.
Small (Weinheim an Der Bergstrasse, Germany)
|November 24, 2024
Summary
New dual-triphase scaffolds (DTSs) significantly enhance rotator cuff tendon-bone repair. These microfibrous implants promote stem cell viability and improve biomechanical properties, offering a promising solution for chronic rotator cuff injuries.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Orthopedic Surgery
Background:
- Rotator cuff injuries lead to chronic pain and functional deficits.
- Retears and scar tissue formation impede effective enthesis repair.
- Current treatments have limitations in restoring native tendon-bone integration.
Purpose of the Study:
- To assess the efficacy of electrohydrodynamic-printed microfibrous dual-triphase scaffolds (DTSs) for rotator cuff enthesis repair.
- To evaluate the biological and biomechanical outcomes of DTS implantation in vitro and in vivo.
- To explore the potential of DTSs in improving clinical outcomes for tendon-bone healing.
Main Methods:
- Fabrication of polycaprolactone-based DTSs with nanohydroxyapatite, nano-magnesium-oxide, and kartogenin.
- In vitro assessment of stem cell viability and differentiation marker expression (TNMD, SOX-9, RUNX-2, SCX).
- In vivo evaluation of repaired enthesis biomechanical properties and lipidomic analysis.
Main Results:
- Scaffolds supported >95% stem cell viability in vitro.
- Enhanced expression of tendon, chondrocyte, and osteoblast markers observed.
- Significant improvement in biomechanical properties (27.0 ± 4.2 N failure load) and modulated phospholipid profiles in vivo.
Conclusions:
- DTSs demonstrate significant potential for enhancing rotator cuff enthesis repair.
- The scaffolds promote cellular differentiation and improve biomechanical strength.
- DTSs offer a promising foundation for personalized strategies in tendon-bone regeneration.
