"Mending with silk" enhances aged silk with mechanical and antibacterial properties

The reinforcement and preservation of historical silk are crucial for its continued research, heritage, and display. Herein, silk fibroin (SF) and carboxymethyl chitosan (CMCS) were utilized as raw materials to reinforce aged silk via two reinforcement methods, with transglutaminase (TGase) serving as a catalytic cross-linking agent. The covalent and non-covalent bond network formed by TGase catalytic cross-linking significantly improved the mechanical properties of aging silk, and CMCS as an antibacterial material gave excellent antibacterial properties of the aged silk. Compared with aged silk, the breaking stress and elongation at break of the aged silk after reinforcement are increased by 257.9% and 293.7% respectively. The antibacterial rate of the reinforced aged silk against Escherichia coli (E. coli) and Streptococcus aureus (S. aureus) exceeded 90%. Under accelerated simulated aging conditions, the aging rate of the reinforced silk was significantly reduced, demonstrating notable aging resistance. Notably, the one-step enhanced aged silk outperformed the two-step enhanced aged silk in all performance evaluations, while the appearance of the aged silk was not affected. This work provides a green and efficient process for the reinforcement and protection of silk-based cultural relics and the application of silk cultural relics protection materials.