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Novel selective and sensitive electrochemical sensor for detection of ovarian cancer biomarker, Cathepsin L

  • 0Amity Institute of Biotechnology, Amity University, Rajasthan 303002, India.
Bioelectrochemistry +

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November 24, 2024

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November 24, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Early ovarian cancer detection is crucial for patient survival. A new electrochemical sensor rapidly detects Cathepsin L, an early biomarker, enabling point-of-care diagnostics for improved outcomes.

Area Of Science

  • Biomedical Engineering
  • Analytical Chemistry
  • Oncology

Background

  • Late detection of ovarian cancer significantly reduces patient life expectancy.
  • Current diagnostic methods lack rapid, point-of-care capabilities for epithelial ovarian cancer.
  • Cathepsin L is identified as a key early-stage biomarker for ovarian cancer progression.

Purpose Of The Study

  • To develop a novel, label-free electrochemical diagnostic tool for sensitive and selective detection of Cathepsin L in serum.
  • To establish a rapid point-of-care diagnostic method for early ovarian cancer screening.
  • To validate the performance of the proposed diagnostic platform using clinical samples.

Main Methods

  • Immobilization of Cystatin C onto a screen-printed carbon electrode using EDC/NHS cross-linking.
  • Detection of Cathepsin L via electrochemical techniques including cyclic voltammetry, differential voltammetry, and electrochemical impedance spectroscopy.
  • Confirmation of probe-analyte interaction using intrinsic fluorescence spectroscopy.

Main Results

  • The electrochemical sensor achieved a limit of detection of 70 pg mL⁻¹ for Cathepsin L using electrochemical impedance spectroscopy.
  • The diagnostic platform demonstrated high reproducibility, selectivity, and stability within a 20-minute detection timeframe.
  • Validation with spiked serum samples showed a strong correlation (R² = 0.98), confirming reliable performance.

Conclusions

  • The developed label-free electrochemical sensor offers a sensitive and selective method for point-of-care detection of Cathepsin L in serum.
  • This novel diagnostic approach holds potential for early ovarian cancer diagnosis and improved patient management.
  • The rapid and reliable performance of the sensor supports its application in clinical screening settings.

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