Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantification of tryptamine in brain using high-performance liquid chromatography.

A A Larson, N L Dalo

    Journal of Chromatography
    |February 14, 1986
    PubMed
    Summary

    Researchers developed a new method to measure tryptamine (a key indoleamine) in rat brains. This technique revealed that certain drug pretreatments significantly increase tryptamine levels, offering insights into neurotransmitter regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evidence for the modulation of nociception in mice by central mast cells.

    European journal of pain (London, England)·2017
    Same author

    Mechanisms of induction of persistent nociception by dynorphin.

    The Journal of pharmacology and experimental therapeutics·2001
    Same author

    Neuronal nitric oxide synthase (nNOS) mRNA is down-regulated, and constitutive NOS enzymatic activity decreased, in thoracic dorsal root ganglia and spinal cord of the rat by a substance P N-terminal metabolite.

    The European journal of neuroscience·2001
    Same author

    Nociceptive aspects of fibromyalgia.

    Current pain and headache reports·2001
    Same author

    Intrathecal Zn2+ attenuates morphine antinociception and the development of acute tolerance.

    European journal of pharmacology·2000
    Same author

    Transient changes in the synthesis of nitric oxide result in long-term as well as short-term changes in acetic acid-induced writhing in mice.

    Pain·2000

    Area of Science:

    • Neurochemistry
    • Pharmacology
    • Analytical Chemistry

    Background:

    • Tryptamine is an endogenous indoleamine found in the central nervous system.
    • Accurate quantification of tryptamine is crucial for understanding its physiological and pathological roles.
    • Existing methods may lack the sensitivity or specificity required for precise measurement in complex biological tissues.

    Purpose of the Study:

    • To develop and validate a sensitive analytical method for determining endogenous tryptamine concentrations in central nervous system (CNS) tissue.
    • To measure basal tryptamine levels in the whole brain of normal rats.
    • To investigate the effect of specific pharmacological pretreatments on brain tryptamine concentrations.

    Main Methods:

    • Tissue extraction using ethyl acetate.

    Related Experiment Videos

  • Purification utilizing a weak cation-exchange resin.
  • High-performance liquid chromatography (HPLC) with fluorometric detection for analysis.
  • Chromatographic separation on a muBondapak C18 reversed-phase column under isocratic conditions.

    • Main Results:

    • The developed method accurately quantified tryptamine in rat brain tissue.
    • Basal tryptamine concentration in normal rat whole brain was determined to be 0.60 +/- 0.06 ng/g.
    • Pretreatment with dl-p-chlorophenylalanine, tranylcypromine, and l-tryptophan resulted in a substantial increase in tryptamine concentration to 96.7 +/- 21.9 ng/g.

    Conclusions:

    • The established HPLC method provides a reliable means for measuring endogenous tryptamine in CNS tissue.
    • Pharmacological manipulation can dramatically elevate brain tryptamine levels.
    • These findings contribute to the understanding of tryptamine's role and its modulation within the central nervous system.