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Updated: Jun 19, 2026
Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
Published on: September 11, 2015
Bioinspired adhesive polydopamine-metal-organic framework functionalized 3D customized scaffolds with enhanced
Jieliang Shi1, Zhirong Liu1, Xiaobin Ren1
1National Clinical Research Center for Ocular Diseases, National Engineering Research Center of Ophthalmology and Optometry, School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, 270 Xueyuan Xi Road, Wenzhou 325027, China.
Abstract:
Critical-sized orbital bone defects can lead to significant maxillofacial deformities and even eye movement disorders. The challenges associated with these defects, including their complicated structure, inadequate blood supply, and limited availability of progenitor cells that hinder successful repair. To overcome these issues, we developed a novel approach using computer numerical control (CNC) material reduction manufacturing technology to produce a customized polyetheretherketone (PEEK) scaffold that conforms to the specific shape of orbital bone defects. Deferoxamine (DFO) was in situ encapsulated into polydopamine-hybridized zeolitic imidazolate framework-8 (pZIF8-DFO) nanoparticles, which was subsequently adhered to the sulfonated PEEK (sPEEK) scaffold through polydopamine modification. This functionalization enhanced drug loading efficiency and imparted anti-inflammatory properties to the nanoparticle system. Our in vitro findings demonstrated that the sustained release of DFO from the sPEEK/pZIF8-DFO scaffolds extended over 14 days and significantly promoted angiogenesis and progenitor cell recruitment, as evidenced by increased expression of HIF-1α, VEGF, and SDF-1α expression in human umbilical vein endothelial cells (HUVECs). Moreover, sPEEK/pZIF8-DFO scaffolds exhibited superior immunomodulation and osteogenic differentiation capabilities on Raw 264.7 cells and rabbit bone marrow mesenchymal stem cells (rBMSCs), respectively. Most notably, our in vivo rabbit orbital bone defects revealed that sPEEK/pZIF8-DFO scaffolds resulted in a greater volume of new bone formation than on sPEEK and sPEEK/pZIF8 scaffolds, with partial bone connection to the sPEEK/pZIF8-DFO scaffolds. In summary, we develop a novel PEEK scaffold that combines enhanced angiogenesis, stem cell recruitment, immunomodulation, and osteogenic differentiation, showcasing its promising potential for orbital bone reconstruction.
