Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simplified lysed-blood culture technique.

C H Zierdt

    Journal of Clinical Microbiology
    |March 1, 1986
    PubMed
    Summary

    A novel blood culture system uses lysing agents like digitonin and Triton X-100 to improve pathogen detection. This method offers lysis filtration and centrifugation benefits without added complexity or cost.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Serum antibody detected by fluorescent antibody test in patients with symptomatic Blastocystis hominis infection.

    Recenti progressi in medicina·1995
    Same author

    Enzyme-linked immunosorbent assay for detection of serum antibody to Blastocystis hominis in symptomatic infections.

    The Journal of parasitology·1995
    Same author

    Lipid biosynthesis by axenic strains of Blastocystis hominis.

    Comparative biochemistry and physiology. Biochemistry and molecular biology·1994
    Same author

    Detection of microsporidian spores in clinical samples by indirect fluorescent-antibody assay using whole-cell antisera to Encephalitozoon cuniculi and Encephalitozoon hellem.

    Journal of clinical microbiology·1993
    Same author

    Antibody response to Blastocystis hominis infections.

    Annals of internal medicine·1993
    Same author

    Taxonomic status of Blastocystis hominis: reply.

    Parasitology today (Personal ed.)·1993

    Area of Science:

    • Microbiology
    • Clinical Diagnostics

    Background:

    • Conventional blood culture methods can be limited by bacterial inhibition.
    • Development of improved lysis techniques is crucial for enhanced pathogen recovery.

    Purpose of the Study:

    • To develop and evaluate a novel blood culture system incorporating lysing agents.
    • To assess the efficacy and potential advantages of this new system compared to existing methods.

    Main Methods:

    • Testing various lysing agents (digitonin, Triton X-100, polyoxyethylene compounds, Tween 20) for blood lysis and bacterial inhibition.
    • Comparative culture studies using bacteremic rabbit blood with conventional, Isolator, and the new lysing medium systems.

    Main Results:

    • Several compounds, including digitonin and Triton X-100, effectively lysed human blood at 0.05% concentration.
    • Some lysing agents showed no inhibitory effect on tested pathogens.
    • Triton compounds exhibited inhibitory effects on sensitive bacteria like Streptococcus pneumoniae and Neisseria meningitidis.

    Conclusions:

    • The developed lysing blood culture system offers potential advantages in lysis filtration and centrifugation.
    • This new system may provide enhanced diagnostic capabilities without increased cost or complexity.

    Related Experiment Videos